After extracting my industrial fruit waste (dried 50g in 100ml distilled water) and measuring its TPC. I went straight into assessing its antibacterial activity. My extract is in liquid form (no rotary evaporation/lyophilization/spray drying). After quick well diffusion tests, I attempted determination of MIC (microbroth dilution method) with Mueller-Hinton broth in U-bottom 96-well microplates.

I then came to realize that I don't exactly know the concentration of my extract. Thus, my question is could I use the mg GAE/ml as the concentration? or is my concentration 50g/100ml i.e. 500mg/ml? or should I dry in the oven (100-120oC) overnight 1ml of my extract and calculate the weight difference per 1ml as my concentration?

Thanks

Edit for anyone interested: After several adjustments, the antibacterial assessment will follow with considering the waste in its "crude" extract state and assessing its MBC/MIC ratio with the literature.

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