Using a fixed angle rotor with Sucrose gradient /cushion ultracentirfugation?

I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...

13 January 2021 8,372 3 View

Can I leave blot in block buffer for 48 hours?

Is it ok ti leave the blot in non fat milk for 48 hours in +4 degrees or is it better to leave it tbs-t for that extra 24hrs?

23 February 2020 7,475 2 View

Should the HCV Core protein fused with His-tag (without linker) be extracted in the insoluble or soluble protein fraction?

I have infected insect cells SF9 with recomb. baculovirus expressing Core.His and another with GFP. I have extracted the soluble proteins after 3 days and did western blot using anti-his,...

10 February 2020 2,359 4 View

in concrete application Which more benefit adding Nano Carbon tube or Nano cellulose from economic side and performance?

My dear colleagues, in concrete application which more benefit adding Nano Carbon tube or Nano cellulose from economic side and performance?

19 April 2019 4,501 8 View

What is the best way to of the waste plastic fiber to enhance some of the mechanical properties of concrete?

Dear Colleagues What is the best way to enhancing the shape and the outer surface of the waste plastic fiber to enhance some of the mechanical properties of concrete?

21 September 2018 7,603 1 View

Which is more useful in improving the impact resistance of the concrete. The use of aluminum waste as an alternative to sand or cement?

Dear Colleagues Which is more useful in improving the impact resistance of the concrete. The use of aluminum waste as an alternative to sand or cement?

31 May 2018 5,727 6 View

Polymer diposition on nanoparticles?

I wanted to diposite a polymer on nanoparticles,but the TEM results shows that that the polymer diposite on an aggregated nanopartilces together. is that a problem of the nanoparticles dispertion...

04 April 2017 204 5 View

How I can calculate vertex coordinates from vertex normal in triangular mesh ?

I have triangle mesh and calculate normal of triangles then calculate vertex normal and do some calculations on it and want to calculate vertex coordinates from this vertex normal after do...

01 November 2016 992 3 View

How to measure catalase activity in a plant extract?

can you describe please the contents of a simple reaction to measure catalase activity in a plant extract and is it possible to be a water extract or should it be in phosphate buffer? thanks a lot

05 April 2016 9,175 3 View

Standards in ELISA??

28 February 2016 9,668 3 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View