Using a fixed angle rotor with Sucrose gradient /cushion ultracentirfugation?

I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...

13 January 2021 8,372 3 View

What is the sampling technique used in an online survey?

I am asking about the sampling technique used in an online survey. The survey will be distributed online via social media channels. We are targeting adolescents. The sample size will be estimated...

11 July 2020 8,685 14 View

Can I leave blot in block buffer for 48 hours?

Is it ok ti leave the blot in non fat milk for 48 hours in +4 degrees or is it better to leave it tbs-t for that extra 24hrs?

23 February 2020 7,475 2 View

Should the HCV Core protein fused with His-tag (without linker) be extracted in the insoluble or soluble protein fraction?

I have infected insect cells SF9 with recomb. baculovirus expressing Core.His and another with GFP. I have extracted the soluble proteins after 3 days and did western blot using anti-his,...

10 February 2020 2,359 4 View

What the best solvent for polystyrene other chloroform?

10 November 2016 5,045 1 View

Analyzing a gaussian output

Hi everyone I did an opt+fre calculation using gaussian 09 , now I have the output file but I don't know how to analyze and interpret my results (HOMO-LUMO and NBOs ) can anyone help?

01 January 1970 8,907 4 View

How can I view my work " paper" and save my idea, while the publishing process takes a long time

If I have a new work and isn't submitted yet to any journal or conference. can I put it in arXiv and what is the advantage and disadvantage of this step?

01 January 1970 3,209 3 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

How do you obtain ASI (Annual Survey of Industries) plant identifiers, to create a panel data?

Dear all, Is there anybody who has worked with ASI (Annual Survey of Industries) Firm-Level data? I have extracted the data files with STATA, and now I need help with creating a panel for...

11 February 2021 876 4 View

Do we become better with vaccination from covid in 2021?

We hope to recover, and the economy improved by using vaccination .and vaccines is needed. The vaccine will protect you from getting ill and then ending up hospitalized. But it's possible that...

02 January 2021 4,194 4 View

Anyone with access to micro-Level data on French Banks?

I am looking for data on French banks from June 2011 to June 2019 - ROA, NIM, ROE, and total equity including loans and assets, preferably from S&P Global, or BvD Bank Focus. Any help is greatly...

06 December 2020 9,930 1 View

How can I find the best subject between architecture, economy, historical site ?

Hi there, I consider to write my final thesis in master architecture. I am trying to find a good link between economy impact , historical site and high rise buildings which I am interested in, but...

16 November 2020 4,682 16 View

How to measure the penetration of AI and ML in an Economy?

Is there any database on Indian economy measuring the use of AI and ML state-wise or at the national level?

30 October 2020 4,402 3 View

What is the effect of COVID-19 on agriculture specifically the prolonged lockdown that the farmers of whole world have faced?

I want to know how it is affecting agriculture around the globe. Because its a platform in which people from different parts of the world unites.

24 September 2020 400 27 View

Do you think "Hydrogen Economy" has future? What happened to the US big plans for "Hydrogen Economy"?

I do believe that "Hydrogen Economy" has good future. The European Union and Japan have big plans, however the US DOE/EERE conservatively dedicated about $100 million a year in grants. Your...

14 September 2020 3,406 8 View

How do I analyse results and what methodology should I use?

I am writing my dissertation on the effects of Covid-19 on Dubai's economy. I have made a questionnaire with a mix of open ended and closed questions/answers. I have also conducted 10 interviews...

03 August 2020 747 14 View

Can you recommend any publications or authors on "The Impact of the IT&C Sector Upon a National Economy"?

Judging by most stock markets, "Big-tech" seems to be the success story, yet there are surprisingly few academic research papers written on this phenomenon. We are looking for studies and authors...

23 July 2020 5,074 3 View