Nahla Fadl

7 Questions 6 Answers 0 Followers

Questions related from Nahla Fadl

Can I use a handheld refractometer to measure the density of fractions from a sucrose gradient ultracentrifugatio?

I am trying to isolate virus infected plasma from low and high density fractions using sucrose cushion method. Can I use a a handheld/portable refractometer to measure the density of the fractions?

03 February 2021 279 0 View

Using a fixed angle rotor with Sucrose gradient /cushion ultracentirfugation?

I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...

14 January 2021 8,833 3 View

Where Should I add the HIS-Tag in HCV Core gene?

I have a primer that add HisTag (direct w/o linker) to the C terminus of HCV Core (191aa.) I am expressing this gene as a recombinant baculovirus in Insect cells and I want to detect by western...

03 March 2020 9,663 0 View

Can I leave blot in block buffer for 48 hours?

Is it ok ti leave the blot in non fat milk for 48 hours in +4 degrees or is it better to leave it tbs-t for that extra 24hrs?

24 February 2020 7,700 2 View

Should the HCV Core protein fused with His-tag (without linker) be extracted in the insoluble or soluble protein fraction?

I have infected insect cells SF9 with recomb. baculovirus expressing Core.His and another with GFP. I have extracted the soluble proteins after 3 days and did western blot using anti-his,...

11 February 2020 2,546 4 View

Is using Bioanalyzer to detect quality and quantitiy of viral RNA accurate?

I extracted viral RNA samples using Qiagen Viral RNA kit. the samples gave concentrations average 30 ng/ul and purity of average 2 for both 260/280 and 260/230. I shipped the samples using RNA...

21 October 2018 2,044 5 View

What is the best method for adapting SF9 cells to grow on Grace's instead of Ex-cell?

I have been growing SF9 cells on ex-cell media and have been trying to adapt them to grow on Grace's. The media becomes extremely turbid after addition of 25% Grace's and 75% ex-cell. How can I...

01 February 2017 5,305 3 View