Nahla Fadl

4 Questions 5 Answers 0 Followers

Questions related from Nahla Fadl

Using a fixed angle rotor with Sucrose gradient /cushion ultracentirfugation?

I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...

14 January 2021 8,611 3 View

Can I leave blot in block buffer for 48 hours?

Is it ok ti leave the blot in non fat milk for 48 hours in +4 degrees or is it better to leave it tbs-t for that extra 24hrs?

24 February 2020 7,585 2 View

Should the HCV Core protein fused with His-tag (without linker) be extracted in the insoluble or soluble protein fraction?

I have infected insect cells SF9 with recomb. baculovirus expressing Core.His and another with GFP. I have extracted the soluble proteins after 3 days and did western blot using anti-his,...

11 February 2020 2,446 4 View

Is using Bioanalyzer to detect quality and quantitiy of viral RNA accurate?

I extracted viral RNA samples using Qiagen Viral RNA kit. the samples gave concentrations average 30 ng/ul and purity of average 2 for both 260/280 and 260/230. I shipped the samples using RNA...

21 October 2018 1,983 5 View