Hey guys, Can anyone please provide me recipe for PAGE gel, not SDS-PAGE.
Thanks
Sachin
Hello Guys, Im doing a compartmental protein extraction for cytoplasm, nuclear and cell wall proteins . In the protocol I have to use beads based homogenizer. Do these homogenizer destroys cell...
08 September 2018 1,598 5 View
We are looking for different immunocells in cancer research. Can anyone please suggest some current methods, markers fir immunoprofiling.
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I have to isolate different T-cell and B-Cells population from mice tumors. Can anyone please suggest me markers for flow cytometry and immunohistochemistry.
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How I extract only extracellular proteins from tissues.
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Just had this random question, please help.
05 June 2017 4,802 2 View
Hello Guys, Im working on some of the pharmacokinetic parameters of our new drug. Our lab is not PK lab, so I had few basic PK questions. I have inject single dose my compound in mice IP and...
31 December 2016 2,635 9 View
Hello Everyone, I'm working on a heterozygous gene. It have dominant mutant and recessive wide type. So I just had question that can both of these copies can be expressed into...
10 November 2016 2,140 3 View
Hello Everyone, I'm working with Agilent 1220 LC system. The openlab software shows that the pump is idle but its shows the pressure is zero. It looks like pump is not building pressure. Can...
09 October 2016 9,533 9 View
Hello everyone, I have to develop a method to analyze sphingolipids by HPLC. I'm little confused about which solvents can I use for better results.Can anyone please suggest. Thanks, Sachin
09 October 2016 2,515 3 View
Hello Guys, I'm doing the TEM of the nanomicelles. I got two different type of images from same sample and from same grid. One sample haves dark background while other haves bright background....
06 July 2016 4,082 7 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View