Do tissues homogenization destroys cell wall ?

Hello Guys, Im doing a compartmental protein extraction for cytoplasm, nuclear and cell wall proteins . In the protocol I have to use beads based homogenizer. Do these homogenizer destroys cell...

08 September 2018 1,435 5 View

What are best way to do immunoprofiling ?

We are looking for different immunocells in cancer research. Can anyone please suggest some current methods, markers fir immunoprofiling.

10 November 2017 8,199 4 View

What are markers for T- cell and B-Cells ?

I have to isolate different T-cell and B-Cells population from mice tumors. Can anyone please suggest me markers for flow cytometry and immunohistochemistry.

10 November 2017 601 9 View

How to extract extracellular proteins from tissues ?

How I extract only extracellular proteins from tissues.

09 October 2017 9,806 3 View

What happens to your signal in a western blot if you don't use saturating amounts of antibody?

05 June 2017 4,659 2 View

Need help with Pharmacokinetic analysis, is my study compartment or non-compartmental ?

Hello Guys, Im working on some of the pharmacokinetic parameters of our new drug. Our lab is not PK lab, so I had few basic PK questions. I have inject single dose my compound in mice IP and...

31 December 2016 2,310 9 View

Can it be possible that both the DNA copies of a gene are express into protein?

10 November 2016 1,986 3 View

Why Agilent HPLC pump not building pressure??

09 October 2016 9,305 9 View

What type of solvent are better to analyze sphingolipids i.e ceramide, gb3, lacCer etc by HPLC ??

09 October 2016 2,366 3 View

Why the size of the nanomicelles is different ?

06 July 2016 6,908 10 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View