Do tissues homogenization destroys cell wall ?

Hello Guys, Im doing a compartmental protein extraction for cytoplasm, nuclear and cell wall proteins . In the protocol I have to use beads based homogenizer. Do these homogenizer destroys cell...

08 September 2018 1,435 5 View

What are best way to do immunoprofiling ?

We are looking for different immunocells in cancer research. Can anyone please suggest some current methods, markers fir immunoprofiling.

10 November 2017 8,199 4 View

What are markers for T- cell and B-Cells ?

I have to isolate different T-cell and B-Cells population from mice tumors. Can anyone please suggest me markers for flow cytometry and immunohistochemistry.

10 November 2017 601 9 View

How to extract extracellular proteins from tissues ?

How I extract only extracellular proteins from tissues.

09 October 2017 9,806 3 View

What happens to your signal in a western blot if you don't use saturating amounts of antibody?

05 June 2017 4,659 2 View

Need help with Pharmacokinetic analysis, is my study compartment or non-compartmental ?

Hello Guys, Im working on some of the pharmacokinetic parameters of our new drug. Our lab is not PK lab, so I had few basic PK questions. I have inject single dose my compound in mice IP and...

31 December 2016 2,310 9 View

Can it be possible that both the DNA copies of a gene are express into protein?

10 November 2016 1,986 3 View

Why Agilent HPLC pump not building pressure??

09 October 2016 9,305 9 View

Why the size of the nanomicelles is different ?

06 July 2016 6,908 10 View

Why two TEM images of same nanoparticles are different ??

Hello Guys, I'm doing the TEM of the nanomicelles. I got two different type of images from same sample and from same grid. One sample haves dark background while other haves bright background....

06 July 2016 3,934 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View