23 Questions 42 Answers 0 Followers
Questions related from Sachin Khiste
Hello Guys, Im doing a compartmental protein extraction for cytoplasm, nuclear and cell wall proteins . In the protocol I have to use beads based homogenizer. Do these homogenizer destroys cell...
09 September 2018 1,520 5 View
I have to isolate different T-cell and B-Cells population from mice tumors. Can anyone please suggest me markers for flow cytometry and immunohistochemistry.
11 November 2017 691 9 View
We are looking for different immunocells in cancer research. Can anyone please suggest some current methods, markers fir immunoprofiling.
11 November 2017 8,283 4 View
How I extract only extracellular proteins from tissues.
10 October 2017 9,937 3 View
Just had this random question, please help.
06 June 2017 4,738 2 View
Hello Guys, Im working on some of the pharmacokinetic parameters of our new drug. Our lab is not PK lab, so I had few basic PK questions. I have inject single dose my compound in mice IP and...
01 January 2017 2,551 9 View
Hello Everyone, I'm working on a heterozygous gene. It have dominant mutant and recessive wide type. So I just had question that can both of these copies can be expressed into...
11 November 2016 2,072 3 View
Hello everyone, I have to develop a method to analyze sphingolipids by HPLC. I'm little confused about which solvents can I use for better results.Can anyone please suggest. Thanks, Sachin
10 October 2016 2,450 3 View
Hello Everyone, I'm working with Agilent 1220 LC system. The openlab software shows that the pump is idle but its shows the pressure is zero. It looks like pump is not building pressure. Can...
10 October 2016 9,433 9 View
Hello Guys, I'm doing the TEM of the nanomicelles. I got two different type of images from same sample and from same grid. One sample haves dark background while other haves bright background....
07 July 2016 4,010 7 View
Hello Everyone, Im working on the nanomicelles which are soluble in water. I did the particle size analysis of these nanomicelles using laser particle size analyser and the particle size was 5-7...
07 July 2016 6,995 10 View
Hello Everyone, I have synthesized some nanomicelles and using one of the particle size counterI come to know that these particles have average size of 5-6 nm ( But also haves some nanoparticles...
06 June 2016 6,001 11 View
Hello Guys, I'm working on MALDI-TOF for the p53 in OVCAR-3 and A2780 cells. I just want to what is percentage of p53 in total protein in these cells or any other cells. Thanks, Sachin
06 June 2016 9,580 3 View
Hello Guys, I have to buy p53 protein for the LC-MS analysis as standard. Can anyone knows any supplier? Thanks Sachin
05 May 2016 5,491 2 View
Hell Guys, I did the IP for the separation of specific protein (p53) form the total protein. Now I have antibodies with my specific protein. To remove antibodies and get my protein I have to do...
05 May 2016 4,757 2 View
Hey guys, Can anyone please provide me recipe for PAGE gel, not SDS-PAGE. Thanks Sachin
05 May 2016 7,297 0 View
Hello everyone, I have one research problem. I have to separate p53 (MW 53 Kda ) from total protein extracted from cancer cells and I have to study it by LC-MS. I have separated the 53 Kda...
05 May 2016 3,374 7 View
Just want to know what is this assay and how its done. Thanks
04 April 2016 7,729 0 View
Hello Everyone, I facing a problem in my research work. To solve that problem I have to transfect the wild-type p53 cells A2780 with the vector containing missense mutant p53 (248). Can anyone...
03 March 2016 7,068 4 View
Hello everyone, I have a huge problem. I have did some p53 studies using wild type p53 cells A2780 and p53 mutant cell lines OVCAR 3 and collected huge data. The problems is one of the reviewer...
02 February 2016 8,371 1 View
Dear All, Im working with Ovcar-3 cell lines. When I thaw the new vail the cells are floating, they are not attachng. To make sure there is no contamination, I tried fresh medium, different...
09 September 2015 8,737 6 View
I am adding 0.1 or 0.2 mg/ml concentration of SPIONs to MCF 7 cells, and 6 and 9 well plates for 2 different assays. But I don't know what volume of these nanoparticles I have to add. Can anyone help?
08 August 2013 3,921 1 View
I did the synthesis of SPION by the following method: Briefly, 0.71 g of iron acetylacetonate was mixed with 20 mL of phenyl ether, 2 mL of oleic acid, and 2 mL of oleylamine under inert...
06 June 2013 9,947 6 View
