I intend to perform cytokine measurements in rodent samples and would like to use the bead based cytokine multiplexing analysis, preferably using the Bio-PlexTM Pro Multiplex Immunoassays. If there is someone who has used this assay before, particularly for rodent serum samples, I would like to know how sensitive this assay is and what is the minimum sample size required to obtain reliable and possibly statistically significant quantification of serum cytokines.
The company should provide sensitivity for each cytokine and it will vary among cytokines. Serum levels are independent of assay used and vary widely between cytokines, chemokines and growth factors. Control levels versus experimental group levels for each biologic can differ by log(s) and it is good to establish baseline levels for each cytokine and group before undertaking an analysis of all the samples. It is common to have to use different serum dilutions for each biologic. Based on the preliminary levels you should be able to reduce effort and consumables.
I know that these are general comments but maybe critical to your efforts.
Hi Mallu Abhiram Charan Tej
I use BD™ Cytometric Bead Array (CBA) Mouse kits and most of these kits are highly sensitive, so a sample size of (n=6) should be sufficient to get a statistically significant data in the serum samples and another advantage is it uses a very sample amount of serum for the assay so we can always confirm the same with ELISA in a few samples.
Thanks,
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