I have a dataset of different taxa of five different site with respect to environment fluctuation? the top contributing taxa (sharing highest % of dissimilarity index ) is sensitive to fluctuations or resistant?
Hi, I am doing aptamer based electrochemical biosensor. First, I cleaned screen printed gold electrode using 0.5M H2SO4. Then, immobilized aptamer followed by blocking using 2mM MCH. I use...
06 July 2024 9,852 1 View
Usually, I use cDNA and perform gradient PCR for primer standardization. What kind of sample cDNA do I have to use in this case and what steps do I need to consider for standardization?
26 May 2024 8,124 0 View
I want to quantify low cAMP levels generated of a constitutively active GPCR also by different mutants of this receptor. I have tried the cAMP ELISA kit from Cayman chemicals with very...
14 April 2024 7,318 0 View
Hello everyone, I am conducting molecular docking using Autodock Vina. The receptor (protein) is huge therefore the grid spacing 0.375 A is not enough (Autodock Tools). So, I also increased the...
22 March 2024 8,367 1 View
That forces are the particular atoms/molecules forces due the effect of another atoms/molecules
10 March 2024 5,464 3 View
Hi everyone, I have expressed 6x histidine tagged protein(35kDa) and purified using Ni-NTA agarose (Qiagen). The elution buffer contain 20mM of Sodium phosphate, 500mM NaCl and 250mM Imidazole....
20 December 2023 7,080 1 View
Hi everyone, I am currently working on protein expression and purification. Transformation was successful using Lemo21 E.coli and plasmid pRSETA. Single colony was inoculated into 100mL LB broth...
18 November 2023 136 6 View
As of now, there is no public database available for this kind of sample to take as a control.
15 October 2023 6,907 3 View
I intend to perform cytokine measurements in rodent samples and would like to use the bead based cytokine multiplexing analysis, preferably using the Bio-PlexTM Pro Multiplex Immunoassays. If...
06 August 2023 4,117 2 View
Majorly used geometries for any storage purposes are cylindrical and spherical geometries depending on state of fluid, is there any other geometry which can be efficient than both the existing...
19 April 2023 6,432 0 View
i am unable to interpret why its increases in start as shown in figure
11 August 2024 2,175 1 View
How can I interpret the data gathered without solving?
03 August 2024 9,052 3 View
I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?
26 July 2024 4,740 1 View
While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...
25 July 2024 9,229 1 View
Hi, I am currently a upcoming 4th year student who is need of your help as I couldn't find any accessible file for the manual scoring and interpretation for the PBOI - White Campbell. My group and...
23 July 2024 2,268 1 View
Hello!!! I want to implement the Swerling characteristics functions (CF) directly in MATLAB without using its Fourier integral pairs...the Swerling CFs are actually Laplace Transform of the signal...
23 July 2024 4,921 1 View
This is a analysis on IHC scoring of WNVs and USUV performed with culex pipiens
22 July 2024 1,880 0 View
Hello, I'm planning a shell exchanging experiment with two marine, hermit crab species inside of a tank. I only have one tank available for this experiment. I plan on running 30-40 shell...
20 July 2024 5,675 3 View
Hi guys, in the context of my master thesis i analyze the statistical relationship between income and subjective well-being (Panel: SOEP, n: 300.000 observations over 10 years). After creating a...
13 July 2024 7,538 6 View
btw have u ever know about math bounded and reality bounded? for example it happen when student interpret the solution of problem toward mathematics. if you have experience or research about that,...
03 July 2024 6,631 2 View