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Questions related from Mallu Abhiram Charan Tej
I intend to perform cytokine measurements in rodent samples and would like to use the bead based cytokine multiplexing analysis, preferably using the Bio-PlexTM Pro Multiplex Immunoassays. If...
07 August 2023 4,083 2 View
Does anyone has experience in quantifying tube length and branching points in H&E images of matrigel plugs using angioanalyzer of imageJ or any other freely available software?
03 February 2022 6,825 1 View
If the required volume of flow-cytometry antibody (conjugated) is too small to measure, is it preferable to pre-dilute the antibody before adding it to the sample? If so, what is the best way to...
15 April 2015 7,485 5 View
We recently started working with human osteoblast cell line SAOS2. We froze the cell line in 15% Mc Coy's 5A medium (1 ml) with 5% DMSO for cryo preservation. after trypsinizing and adding the...
03 July 2014 2,639 5 View
I am trying to measure nitrite content in serum samples by Griess assay. But I am facing problems like curdling of serum (possibly due to plasma contamination). Do I have to do some pre-treatment...
07 August 2013 6,651 1 View
We are facing a problem in induction of hind limb ischemia in SCID mice. We tried common femoral artery ligation (only knot) and also cut, but it is not inducing ischemia as expected. The limb is...
05 June 2013 6,493 15 View
I would like to have a software to manage large amounts of clinical data.
03 May 2013 5,624 3 View
If I want to patent an invention, can I discuss (poster presentation) in a scientific meeting regarding the same prior to patenting it ? In other words, if once presented in a scientific meeting,...
23 January 2013 9,464 8 View
We are having a problem in detecting the basal eNOS levels in freshly isolated PBMCs by RT-PCR or western blotting. Does anyone have suggestions for the same and a maybe a protocol?
09 January 2013 9,931 2 View
