Dear fellas,
When I read literature, I came across this: "RNA stabilisation solution [0.2% SDS, 19% ethanol, 1% acidic phenol in water]. Regarding the phenol, does it mean that if I prepare 100 ml RNA stablization solution, I need to add 1 ml of Water-saturated phenol (so that the final concentration is 1% for the acidic phenol in water)?
Theoritically, this implies,
0.2% SDS = 0.2g SDS + distilled water and dilute to 100 ml mark.
1% acidic phenol in water = 1g of phenol crystal in distilled water and dilute to 100 ml mark.
19% ethanol = 19ml of ethanol and dilute to 100 ml mark with distilled water.
But since no specific mixture ratio is given, practically, you measure 0.2 g SDS + 19 ml of ethanol + 1 g of phenol + distilled water and makeup to 100 ml mark.
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