fungal inoculum treated with lacto phenol blue and observed under microscope anyone help me to identify and share some information regarding this fungal slide
Desalegn Amenu
Culture Characteristics: Observing the growth pattern, colony morphology, and sporulation on culture media like potato dextrose agar (PDA). Microscopic Examination: Detailed examination of the sporangia, sporangiospores, and the presence of rhizoids (root-like structures that Rhizopus produces at the base of sporangiophores). Molecular Identification: DNA sequencing of specific genes (such as ITS region) for accurate species identification.
Possible Species:
Given the general appearance and the structures visible in the image, the most likely species include:
- Rhizopus stolonifer: Commonly known as black bread mold, it is one of the most widespread species of Rhizopus. It typically has large black sporangia and grows rapidly.
- Rhizopus oryzae: Often found in fermented foods and soil, and also has large sporangia but may have slightly different growth characteristics compared to R. stolonifer.
Confirmation:
For a precise identification, further steps would include:
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