Cannot open big files in GraphPad Prism?

Good morning. I have recently switched computers and gotten a new edition of Graphpad. I am trying to open a very large file containing all of my graphs for a project, but am encountering an...

22 March 2020 6,810 1 View

Sorting negative cells from GFP dim parental, please help?

I have an established cell line that expresses GFP constitutively. Once I transfect the cell line, there shouldn't any GFP expression since the DNA payload doesn't have a fluorescence marker. My...

23 September 2019 3,940 1 View

FACS-related Co-op project, can someone help me with their input?

Good evening - I need to come up with a project idea for co-op. The project needs to be mostly FACS-related but also give exposure to other techniques, such as cell culture and molecular biology...

18 September 2019 8,911 2 View

How do I run moderation with repeated samples and no experimental manipulation?

Hi everyone! I was wondering if anyone might have any ideas of how to conduct some potential analyses for some research I am working on. I am interested in the moderating role of trait emotion...

09 May 2019 5,118 3 View

How to link SAP2000 to matlab?

About connecting SAP with matlab

16 November 2018 2,663 3 View

How to link opensees to matlab?

I want to link matlab to opensees to complete the dynamic stability analysis of a 2D frame

13 August 2018 3,721 4 View

I want to measure the level of the tlr4 in tumor tissue, which method is better Immunohistochemistry vs western blot?

Dear all, Anybody could explain to me which method is better for measurement the level of toll like receptor protein in tumor tissue , Immunohistochemistry vs western blot? Thanks in advance.

09 April 2018 6,343 5 View

What is the pH to reach in measuring titratable acidity?

Dear all, For measuring the titratable acidity, I see titration of sample with 0.1 N NaOH to reach specific pH and reported by lactic acid. But the specifi pH I see is different in literiture,...

15 March 2018 8,141 3 View

Any tips for rodent sperm isolation/RNA extraction from sperm?

My lab is interested in extracting RNA from sperm (small RNAs specifically), and I believe we have the correct kit (Zymo Quick-RNA Micro-prep), but we already have a lot of trouble with extracting...

06 August 2017 8,639 1 View

Measuring prebiotic activity of a fruit

05 August 2017 5,723 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Peptide precipitation as pre-step for acid hydrolysis for amino acid analysis?

I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...

14 February 2021 6,049 3 View

What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View

Can you use MTS assay with cells cultured in media containing phenol red?

I am trying to run a cell viability assay with LN229, but these cells are cultured in DMEM which contain phenol red. Is the MTS assay sensitive to the additional of phenol red in the media? If so,...

03 February 2021 9,478 2 View

How to design an Ultrafiltration membrane for a CSTR?

Dear all, I am designing an Ultrafiltration (UF) membrane coupled with a CSTR. It is desired to utilized a dead end stirred reactor. The main purpose is to separate the enzyme used from the final...

27 January 2021 7,924 1 View

How to improve the yield for the reaction below?

I am trying to increase the yield for the reaction below. I did the column and I saw that the major side-product is the symmetric dimer formed by 4-bromobenzyl bromide. I imagine that NaH is...

20 January 2021 7,874 4 View

What do you think about the stability of redox active ketoesters? Can I isolate this compounds?

Although I want to synthesize a redox active ketoester like 1,3-dioxoisoindolin-2-yl 2-oxo-2-phenylacetate, I have no idea about the stability of this kind of compounds. I know that some redox...

19 January 2021 5,672 3 View

Work up of ester?

What is the detailed procedure for work up in ester synthesis from acid chloride and alcohol? What organic solvent to use for extraction?

18 January 2021 8,649 6 View

Methanol usage in determination of vitamin B2 in foods using HPLC?

After acid hydrolysis and incubation in the dark using Taka-diastase enzyme, 30% sulphuric acid was added to stop the enzymatic reaction. The solution was diluted to the mark and some of the...

16 January 2021 3,740 4 View