Walton Cai

3 Questions 3 Answers 0 Followers

Questions related from Walton Cai

What does it mean by "1% acidic phenol in water"?

Dear fellas, When I read literature, I came across this: "RNA stabilisation solution [0.2% SDS, 19% ethanol, 1% acidic phenol in water]. Regarding the phenol, does it mean that if I prepare 100 ml...

12 August 2020 1,614 2 View

What is the metabolism of E. coli during anaerobic growth in rich media, LB or TSA?

Hi fellas, Recently, we are culturing E. coli in Tryptone Soy Broth under anaerobic conditions. I am just wondering how the bacteria produce energy and what is the metabolism under this growth...

14 January 2019 7,994 1 View

5'RACE PCR in E. coli, broken RNA?

Hi all, I am using SMARTer 5RACE kit from takara to find the transcription start site of genes in E. coli. But, the PCR products were not uniform in length, but with different length. Also, the...

08 August 2018 1,428 3 View