I want to know how can i express my absorbance values in nmol/mg protein in a spectrophotometric assay of protein carbonylation assay?

I need a hypothetical example as an answer for my question for a better understanding. After getting the absorbance values of the protein samples by DNPH method, how can i express my results in...

17 March 2018 6,112 3 View

Concentration of control drug in MTT assay

i am treating cells with different concentrations of drug. now that i want to use the drug as a control in MTT assay, what concentration of drug should i keep as a control? plz suggest

26 June 2017 1,215 3 View

Can anyone give me a working protocol for bacterial cells- based MTT assay?

22 June 2017 7,360 3 View

I want to grow a strictly aerobic bacteria in broth tubes. shall i use cotton plugs only or aluminium foil alongside ? please suggest

i am working on bacterium, Deinococcus radiodurans

21 June 2017 9,456 2 View

Is there any appropriate selective media to grow Deinococcus radiodurans ?

19 June 2017 2,217 3 View

How do I do a serum dilution so that I get 20ug of protein for western blot ?

I added loading buffer of 2ul with DTT, and Bromophenol blue with 10ul of serum, but serum solidified after 5 minutes at 95C heating.

10 September 2014 5,888 2 View

Why when initially culture gave a good yield of plasmid now suddenly the yield has come down to 3ng/ul?

I have to proceed for digestion where the plasmid yield is not sufficient.

11 June 2014 9,193 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View