What can be used as loading control or positive control for MBL western blot ?

More Bizaya Malla's questions See All

Which image (SEM, FESEM TEM) will be more precise to calculate the particle size of hydroxyapatite?

I expect the suggestion about how to calculate the particle size of hydroxyapatite extracted from biowastes.

03 June 2021 9,755 14 View

What will be the suitable solvent for chewable tablet of cholecalciferol[60,000 IU]. Its color is sunset yellow lake?

This vitamin will be blended with polycaprolactone to prepare nano fibrous scaffolds for bone tissue engineering

28 October 2018 1,593 1 View

I want to know how can i express my absorbance values in nmol/mg protein in a spectrophotometric assay of protein carbonylation assay?

I need a hypothetical example as an answer for my question for a better understanding. After getting the absorbance values of the protein samples by DNPH method, how can i express my results in...

17 March 2018 6,169 3 View

What about the P- E loop that is occupying 3rd and fourth quadrant instead of 1st and third quadrant? Need expert' s suggestion what and how it is?

Recently we prepared Sr doped YCrO3 whose P- E loop falls in second and fourth quadrand instead of ist and 3rd. NOW the same case occurs for the composite of LaNaCrO3+NiCuFe2O4. Also here the...

01 March 2018 3,282 5 View

What calculation should i go through?

i have a stock solution of 500 milli molar ebselen in 100% DMSo. now i want to keep the final conc of DMSO to 0.1% , keeping in mind that the final conc of the drug (ebselen), is taken to be 1...

28 June 2017 7,333 1 View

Concentration of control drug in MTT assay

i am treating cells with different concentrations of drug. now that i want to use the drug as a control in MTT assay, what concentration of drug should i keep as a control? plz suggest

26 June 2017 1,263 3 View

Can anyone give me a working protocol for bacterial cells- based MTT assay?

i want to treat the bacteria with drugs and then check the viability of the cells

22 June 2017 7,414 3 View

I want to grow a strictly aerobic bacteria in broth tubes. shall i use cotton plugs only or aluminium foil alongside ? please suggest

i am working on bacterium, Deinococcus radiodurans

21 June 2017 9,526 2 View

Is there any appropriate selective media to grow Deinococcus radiodurans ?

since i am trying to grow deinococcus radiodurans in my lab, i encounter a lot of compititive bacteria like E. coli and Bacillus types. please suggest

19 June 2017 2,273 3 View

How do extracellular enzymes get released out of the bacterial cells?

I wanted to know whether there are any mechanisms by which cells release the enzymes. How few of enzymes are retained inside cells and called 'intracellular enzymes', at the same time few others...

07 March 2016 6,016 4 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View