Can anyone give me a working protocol for bacterial cells- based MTT assay?

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Which image (SEM, FESEM TEM) will be more precise to calculate the particle size of hydroxyapatite?

I expect the suggestion about how to calculate the particle size of hydroxyapatite extracted from biowastes.

03 June 2021 9,755 14 View

What will be the suitable solvent for chewable tablet of cholecalciferol[60,000 IU]. Its color is sunset yellow lake?

This vitamin will be blended with polycaprolactone to prepare nano fibrous scaffolds for bone tissue engineering

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I want to know how can i express my absorbance values in nmol/mg protein in a spectrophotometric assay of protein carbonylation assay?

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What about the P- E loop that is occupying 3rd and fourth quadrant instead of 1st and third quadrant? Need expert' s suggestion what and how it is?

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What calculation should i go through?

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Concentration of control drug in MTT assay

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26 June 2017 1,263 3 View

I want to grow a strictly aerobic bacteria in broth tubes. shall i use cotton plugs only or aluminium foil alongside ? please suggest

i am working on bacterium, Deinococcus radiodurans

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Is there any appropriate selective media to grow Deinococcus radiodurans ?

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How do extracellular enzymes get released out of the bacterial cells?

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07 March 2016 6,016 4 View

What are the pros and cons of using HR and respiration rate to measure stress?

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

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How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Martin Klvana

PubMed: https://www.ncbi.nlm.nih.gov/pubmed

Query: "MTT assay"[title/abstract] and "bacteria"[title/abstract]

Article A colorimetric assay for quantitating bovine neutrophil bact...

Debaki Ranjan Howlader

If you're using a kit for that, then you can just follow the supplied protocol.

Illiyas MAQBOOL Malla

since i am gonna give two diferent drug treatments in a single assay, there is a wash step required in between. moreover, my cells are in suspension rather than adherent. this puzzles me how can i wash the cells in a 96 well-plate.