I want to know how can i express my absorbance values in nmol/mg protein in a spectrophotometric assay of protein carbonylation assay?

I need a hypothetical example as an answer for my question for a better understanding. After getting the absorbance values of the protein samples by DNPH method, how can i express my results in...

17 March 2018 6,112 3 View

Concentration of control drug in MTT assay

i am treating cells with different concentrations of drug. now that i want to use the drug as a control in MTT assay, what concentration of drug should i keep as a control? plz suggest

26 June 2017 1,215 3 View

I want to grow a strictly aerobic bacteria in broth tubes. shall i use cotton plugs only or aluminium foil alongside ? please suggest

i am working on bacterium, Deinococcus radiodurans

21 June 2017 9,456 2 View

Is there any appropriate selective media to grow Deinococcus radiodurans ?

19 June 2017 2,217 3 View

What can be used as loading control or positive control for MBL western blot ?

Western blot, serum sample

12 September 2014 3,212 2 View

How do I do a serum dilution so that I get 20ug of protein for western blot ?

I added loading buffer of 2ul with DTT, and Bromophenol blue with 10ul of serum, but serum solidified after 5 minutes at 95C heating.

10 September 2014 5,888 2 View

Why when initially culture gave a good yield of plasmid now suddenly the yield has come down to 3ng/ul?

I have to proceed for digestion where the plasmid yield is not sufficient.

11 June 2014 9,193 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View