since i am trying to grow deinococcus radiodurans in my lab, i encounter a lot of compititive bacteria like E. coli and Bacillus types. please suggest
Selective conditions . . .
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"Description of the type strain of [Deinococcus] radiodurans nom. nov. (...) Strictly aerobic. Optimal growth temperature is 30°C. All strains grow in the presence of 1% NaCl and most grow in 5% NaCl in media. (...) Resistant to 1.5 Mrad of gamma radiation and exposure to 1,800 ergs/cm2 per s of ultraviolet radiation for 10 min."
"Description of Deinococcus gen. nov. (...) Carotenoid pigments usually are present, and colonies are usually pink to brick red."
Article Nomenclature for "Micrococcus radiodurans" and Other Radiati...
Thank you Martin . your answer will be really helpful. but alongside i want to know that how much DL- methionine should i add to 1000 ml of culture media. i checked a lot of the stuff about it but all have given different views and concentrations. please suggest.
The paper below uses 1 mM methionine, but, as the paper demonstrates, methionine is not necessary . . .
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Not an experimentalist.
Article Development of a defined medium supporting rapid growth for ...
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