i am treating cells with different concentrations of drug. now that i want to use the drug as a control in MTT assay, what concentration of drug should i keep as a control? plz suggest
Assuming a cell culture with different drug concentration scenario. It would be better if you start with no drug as control.
If you must, there are different options to choose a particular concentration of drug as control, like, the maximum concentration where drug have no effect on viability of cells, next it can be the IC50 of drug or the minimum concentration of drug which kills 100% cells.
However, it all depends on what you want to study and what is your objective. Control is just a standard to normalize results (to a standard point).
moreover, i want to know if it is neccessary to remove MTT dye before sollubilizing formazan with any solvent and taking the absorbance values there after? since my cells are in suspension, i cant remove the dye at all in a 96 well-plate. plz suggest