I see some papers have 10% w/v tween 80 or even more, but if we increase the concentration to 10% doesn't the dissolved drug or the particle get adhered to the tube? and also that might have some effects on the dialysis membrane?
Please correct me if I am wrong.
Thank you
The critical micellar concentration of Tween-80 is about 0.0013% and the micelle molecular weight is 76,000. This means that the Tween-80 is not going to dialyze out of the bag in any reasonable amount of time, using dialysis tubing with a typical molecular weight cutoff (e.g. 10,000) if the starting concentration is as high as 10%. If a small molecule drug associates with the micelles because of its hydrophobicity, it will also not dialyze. Nanoparticles are generally too large to dialyze in any case. Depending on what they are made of, they may be dissolved by the detergent or coated by it.
You should try to keep concentration as low as possible. If you cannot reduce ween 80 concentration due to insolubility of drug, you can reduce amount of drug taken but in detectable range. Another problem that I have faced is that Tween 80 shows peak between 215 - 250 nm which is the same region where my drug shows peak. In such case, you will get false results even if you auto-zero with medium.
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