Dear Karn,

It is better if u mention the crop on which u are working. Most of the woody plants wont respond much for normal tissue culture techniques. For successful anther culture as a first initiative, you have to select the anthers which contain late uninucleate stage microspores. Followed by this one has to check suitable hormonal combination, pre-treatment temperatures (for changing gametophytic pathway to sporophytic path way), type of sugar and concentration, light, genotype etc. for direct embryo formation or embryogenic callus development from microspore. The next stage is embryo germination or regeneration from embryogenic callus. But, in most of the cases, u will get the regenerants from somatic tissues so u have to characterize your regenerants with the use of flow cytometry or root tip squash techniques for haploid screening. 

Dear Kurimella,

I am happy that we are together for a specific reason. I am working on Potato anther culture. Recently, I have been found some fruitful results showing callus in the anther of Momordica Charantia.