What is the proper range of FRET efficacy?

I would like to use FRET effect to probe the distance between two functional moieties (I am sure the distance is smaller than 10 nm). I have tried several times, but I found out that the FRET...

21 February 2021 3,562 1 View

What's the function of each ingredient in a binding buffer?

I read a binding buffer in references. The recipe was listed as follows: Glucose (4.5g/L), Magnesium Chloride (5 mM), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml) was dissolved in DPBS. This buffer...

23 December 2020 8,440 4 View

Why transwell insert can cut off some drug molecules?

I am preparing a co-culture experiment in which transwell inserts will be used. According to the references, 0.4 micrometer PET membrane was selected. And different drugs were added to the upper...

15 November 2020 3,718 2 View

Can I use HUVEC cell line, not primary cell, to form barrier system in transwell?

I would like to measure the permeability of my drug using in vitro model, transwell, specifically. However, I only have the cell line, not the primary cell. Can I use the cell line to plate in the...

08 November 2020 3,330 1 View

How can I choose the concentration of Triton X-100 when I would like to permeabilize cells for flow cytometry?

Triton X-100 has been widely used to permeablize cells for flow cytometry. How can I choose the concentration of Triton X-100 if I would like to detect expression of one protein in the whole cell?...

31 October 2020 462 1 View

How to define Darcy's flow for pore pressure cohesive element in Abaqus?

Hi everyone, I am trying to define a Darcy's flow for pore pressure cohesive element in Abaqus. When I give permeability to the elements, the output of tangential flow rate (GFVR) is always shown...

11 October 2020 1,195 3 View

Extracting surface area, volume and cortical thickness from multiple groups using FreeSurfer?

Hello everyone, I'm new to FreeSurfer and this question might sound a bit stupid: I'm trying to get the volumetric measures, surface area and cortical thickness of brain regions of three groups...

29 July 2020 8,574 3 View

How many cm deepth of soil does the definition of soil composition generally refer to?

In general, for example, we think soil consist of 40% of minerals, 10% of organic matters, and 50% of water and air. My question is as follow: Does the soil used for the above definition refer...

03 July 2020 4,972 1 View

Dose someone could give me sugestions about 3DNA, Nucleic acid builder, Namot2 and PyMol?

I am working on the MD simulation of 3D self-assembling nanostructures. But researchers in our group have mainly worked on proteins, small molecules and synthesized polymers. Softwares including...

31 March 2020 9,688 3 View

How to chose right data form Protein data bank?

I would like to make MD simulation of a 42-bp B-DNA double helix, which has a segment of single strand at each end of the chain, and a small molecule anti-cancer drug. I have searched PDB and NDB...

09 March 2020 3,605 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What condition required for coating of Fe3O4@SiO2 by using APTES?

I tried coating Fe3O4@ SiO2 by using APTES in 5% water-ethanol but it is not coated. Kindly suggest a solution for it. Thanks

24 February 2021 5,992 4 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

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22 February 2021 9,932 11 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

What properties of water shall be analyzed and evaluated to test the effectiveness of activated carbon?

I would like to ask what other properties of water (pH and such) shall be analyzed and evaluated to test the effectiveness of activated carbon from filtering the contaminants present in the water?

20 February 2021 9,422 8 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View