Western blot showed distorted band, reverse 'U' shape, low MW protein?

Pr Mei @Pr-Mei

05 January 2024 0 7K Report

This is 12% SDS PAGE gel and the MW of this protein is around 20 kDa.

Running: 80V 20min; 110V 2h30min

Transfer: 100V 1h20min

I wonder this might be due to the gel over-heated during the running?

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