Can any one explain me what is mitotic index?

09 October 2015 9,745 1 View

I am doing western blotting and the transfer of protein from gel to PVDF membrane is not that muck efficient. How can I trouble shoot it?

Nearlly 5 to 6 times I have done my western blotting, in which not all the protein in my gel is transferred efficiently to PVDF membrane and we use wet transfer method for blotting 100 volts for...

06 July 2014 7,514 10 View

Is it necessary to store pH 6.8 and pH 8.8 tris buffer at 4 degrees?

We use to prepare 1.5M tris pH 8.8 and 1 Mtris ph 6.8 for SDS and we keep it at room temperature. After few days I could see there is a increase in pH

06 July 2014 5,673 14 View

Do ErbB3 have isoforms and if so what are they?

Do they have an important role in cancer?

04 May 2014 6,939 3 View

Why are we quantifying the gene of interest say erbb3 with the housekeeping gene? What is the concept behind it?

And can any one explain to me the delta Ct method that is used to quantify the gene of interest?

04 May 2014 8,823 15 View

What muttation in Rb1 gene leads to retinoblastoma tumor?

For example due to frame shift or point mutation. What type of mutation does occur in RB1 gene that leads to retinoblastoma tumor?

03 April 2014 1,083 2 View

Why do we use marker for PCR amplified cDNA samples?

Generally to know the molecular weight of the unknown sample we add marker, but in this case of cDNA we know the size of the base pair that we are going to amplify so why do we need the marker?

03 April 2014 3,197 6 View

What is the purpose of using two layers of gel in SDS- PAGE that is staking gel and separating gel?

What is the reason for using two separate gel.

03 April 2014 7,170 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View