I am doing western blotting and the transfer of protein from gel to PVDF membrane is not that muck efficient. How can I trouble shoot it?

Nearlly 5 to 6 times I have done my western blotting, in which not all the protein in my gel is transferred efficiently to PVDF membrane and we use wet transfer method for blotting 100 volts for...

06 July 2014 7,514 10 View

Is it necessary to store pH 6.8 and pH 8.8 tris buffer at 4 degrees?

We use to prepare 1.5M tris pH 8.8 and 1 Mtris ph 6.8 for SDS and we keep it at room temperature. After few days I could see there is a increase in pH

06 July 2014 5,673 14 View

Do ErbB3 have isoforms and if so what are they?

Do they have an important role in cancer?

04 May 2014 6,939 3 View

Why are we quantifying the gene of interest say erbb3 with the housekeeping gene? What is the concept behind it?

And can any one explain to me the delta Ct method that is used to quantify the gene of interest?

04 May 2014 8,823 15 View

What muttation in Rb1 gene leads to retinoblastoma tumor?

For example due to frame shift or point mutation. What type of mutation does occur in RB1 gene that leads to retinoblastoma tumor?

03 April 2014 1,083 2 View

Why do we use marker for PCR amplified cDNA samples?

Generally to know the molecular weight of the unknown sample we add marker, but in this case of cDNA we know the size of the base pair that we are going to amplify so why do we need the marker?

03 April 2014 3,197 6 View

What is the purpose of using two layers of gel in SDS- PAGE that is staking gel and separating gel?

What is the reason for using two separate gel.

03 April 2014 7,170 9 View

What are the differences between side population cells (Sp) and cancer stem cells (CSC) with regards to cancer?

Both SP cells & CSC have stemness property in which Sp cells have greater ADCG2 protein expression. Are there any other differences between between Sp cells & CSC?

10 November 2013 9,234 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View