Can any one explain me what is mitotic index?

09 October 2015 9,745 1 View

I am doing western blotting and the transfer of protein from gel to PVDF membrane is not that muck efficient. How can I trouble shoot it?

Nearlly 5 to 6 times I have done my western blotting, in which not all the protein in my gel is transferred efficiently to PVDF membrane and we use wet transfer method for blotting 100 volts for...

06 July 2014 7,514 10 View

Is it necessary to store pH 6.8 and pH 8.8 tris buffer at 4 degrees?

We use to prepare 1.5M tris pH 8.8 and 1 Mtris ph 6.8 for SDS and we keep it at room temperature. After few days I could see there is a increase in pH

06 July 2014 5,673 14 View

Do ErbB3 have isoforms and if so what are they?

Do they have an important role in cancer?

04 May 2014 6,939 3 View

Why are we quantifying the gene of interest say erbb3 with the housekeeping gene? What is the concept behind it?

And can any one explain to me the delta Ct method that is used to quantify the gene of interest?

04 May 2014 8,823 15 View

Why do we use marker for PCR amplified cDNA samples?

Generally to know the molecular weight of the unknown sample we add marker, but in this case of cDNA we know the size of the base pair that we are going to amplify so why do we need the marker?

03 April 2014 3,197 6 View

What is the purpose of using two layers of gel in SDS- PAGE that is staking gel and separating gel?

What is the reason for using two separate gel.

03 April 2014 7,170 9 View

What are the differences between side population cells (Sp) and cancer stem cells (CSC) with regards to cancer?

Both SP cells & CSC have stemness property in which Sp cells have greater ADCG2 protein expression. Are there any other differences between between Sp cells & CSC?

10 November 2013 9,234 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View