Generally to know the molecular weight of the unknown sample we add marker, but in this case of cDNA we know the size of the base pair that we are going to amplify so why do we need the marker?
Hi, Revathi, You always have to control the specificity of your amplification. In some case, even with cDNA, you may got amplification of the fragment different form one you are look for. So, to be 100% sure that you data was correct, you have to now size of the fragments.
Good luck!
It is necessary to avoid "hand" mistakes, I always add DNA ladder to the gel.
Hi, Revathi, You always have to control the specificity of your amplification. In some case, even with cDNA, you may got amplification of the fragment different form one you are look for. So, to be 100% sure that you data was correct, you have to now size of the fragments.
Good luck!
when you amplify your cDNA with specific primers you should get exact amplification theoretically.. but in some case you may get different amplifications (depends on primer efficiency or your cDNA quality). to be more accurate you should add marker!! that doubles your confidence in your observation :)
It's a way to ensure/compare your result with your theorical size amplification and sometimes it could help you to check for undesired genomic DNA amplification. (and if you are using lambda/HindIII marker you will quantify your results) Good luck! ;)
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