I see a new term on RG statistics page "Total Research Interest":What does this interpret?

"Total Research Interest" is some new index. Can someone explain what does this imply? Maybe its a simple entity but iam not aware of this. Regards

01 February 2019 2,837 26 View

No title

31 December 2018 1,881 30 View

Should basic medical college training other allied medical specialties incorporate managerial sciences and information technologies?

The world is changing fast and lot many biotechnologies are now being routinely utilized by medical professionals in all domains. However, they do not have a formal training in this regard....

10 November 2018 2,917 24 View

How do you compare glucometer readings with lab gkucose results?

While both glcometer & lab glucose measuring technologies use different methodologies and sample. Still from physician to medic compare their results. What do you trust more & for what...

10 November 2018 5,017 7 View

I was looking at the publication fee before depositing an article for review. Can anyone explain what these terms mean?

Dear ALL Creative Commons Attribution (CC BY) Creative Commons Attribution-Non Commercial-No Derivs (CC BY-NC-ND) I am not sure what these publication options means.Obviously i intend to pay...

10 November 2018 1,860 16 View

Do you think genomic medicine will replace conventional medicine ?

Genomic data is emerging fast and every something new molecular research data is there. So what is you take on my question?

10 November 2018 7,222 23 View

Should we as healthcare worker waer our white coats/hospital dress outside the hospital?

I believe we are spreading more infection and antibiotic resistance if we are taking/wearing our white coats (the trade mark of ant medic) outside hospital. Should we do that? Are we not spreading...

09 October 2018 3,555 15 View

If you are writing a mini review can you add a scematic from somebody's article after referencing?

This question is about writing a mini review where i want to add a schematic from some article after appropriate referencing.Can i do that?

08 September 2018 5,590 4 View

What is the preferenable bioniformatics base for RNA base alignments?

Google search and different RNASeq experiments highlights many types of RNA bases for read alignments. Can someone suggest a user friendly base fro small micro RNA alignments to known sequences?

07 August 2018 3,011 2 View

Can NAFLD lead to chronic liver disease?

Excessive fat accumulation within hepaocytes seems pathological. Can it be a cause of cirrhosis or chronic liver disease?

07 August 2018 4,953 34 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

SiRNA transfection efficency and results interpretation ?

i am having a problem with the transfection of 2 siRNA , one for the longer isoform U and the other one of the sliced isoform S that is also the only transcribed. i am trasfection siRNA with a...

11 February 2021 8,145 3 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Why do I see variability in mRNA expression in qPCR of the same gene (ubiquitin)?

Hi everyone! I use HaCaT cells and i transfect them with siRNA. After 48 h I extract proteins with Laemmli and RNA with trizol. I proceed with western blot to check proteins levels (and they are...

26 January 2021 5,738 3 View

Measuring small RNA abundance, without sequencing?

Dear all, Do you know any reliable and fast method to measure the levels of plant small RNAs (21,22 and 24 mer)? I am interested in only comparing the abundance of those small RNA between mock...

21 January 2021 4,002 6 View