Hello,
I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that they tend to release moisture with time approximately within 15 days. Additionally, the (silica) desiccant turns pink, indicating the presence of moisture. This is causing issues with the stability and performance of the membranes.
Has anyone encountered similar problems or have suggestions on how to resolve this issue? Specifically:
- Is there a more effective drying method or a different drying temperature that could prevent the release of moisture?
- Are there alternative storage conditions or desiccants that could help maintain the dryness of the membranes?
- Does presence of moisture also affects the migration of blood sample from gold conjugate pad?
Note:
- Silica is activated properly.
- Incubator in not placed in DH
Any insights or recommendations would be greatly appreciated.
Thank you!
Malaria-coated nitrocellulose membranes in rapid diagnostic tests often suffer from moisture-related issues, degrading antibodies or antigens and causing clumping, noise, and damage. To mitigate this, store membranes in dry environments with desiccants, vacuum seal, control temperature and humidity, and use moisture-barrier packaging. Implement rigorous quality control and strict handling protocols to maintain test reliability and integrity.
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