Can members recommend protocols and primers for PCR identification of randomly selected nematodes? A general first sweep method for identification to family or genus.
Can someone suggest some current protocols being used?
Hi Lawrence,
There are universal primers targeting the ITS-2 zone of nematodes rDNA described by Gasser et al. 1993, you can find more information in my papers.
Article Identification of parasitic nematodes by PCR-SSCP of ITS-2 rDNA
Article A database for ITS2 sequences from nematodes
Marine Benthic Nematode Molecular Protocol Handbook (Nematode Barcoding)
https://isa.org.jm/files/files/documents/tstudy7.pdf
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