Why does exogenously applied iron salts cause leaves to become greener under sufficient iron conditions?

Do iron salts increase photosynthesis, or do they increase the size of chloroplasts, or cytochromes when iron is sufficient? In other words, is greening-up functional or structural under these...

26 January 2021 9,584 4 View

Why do my fibroblasts under hypoxia conditions proliferate slower than the same fibroblasts under normoxic conditions?

I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...

12 January 2021 6,260 1 View

Mitigating future academic stressors during pedagogical development and instructional design?

Greetings from Vancouver, Canada! My doctoral research is going to begin soon. Because the instance of higher education students in Canada, and elsewhere, has been on the rise for more than a...

11 November 2020 2,600 3 View

How do you calculate the substrate concentration of an assay in enyzme kinetics given the volume and the buffer volume at a given 6mM of the enyzme?

In an attempt to plot the michaelis- menten curve, it requires the initial rate as the y-axis and the substrate contration as the x-axis. Now how do you get the substrate concentration of the...

25 October 2020 1,702 3 View

How to crystallize curcumin from ethanolic turmeric extract?

I'm having difficulty recovering curcumin from my ethanolic extract. I tried reducing its solubility in the solution by slowly adding water, but the solid just crashed out of the solution in very...

03 September 2020 3,020 5 View

Trace metal speciation?

I am carrying out a research to assess the ecological risk associated with the speciation of trace and toxic metals in floodplain suspension. Bearing in mind that the reducible, oxidizable and...

18 August 2020 4,333 4 View

What technique can be used for imaging wet hydrogels?

I'm working on alginate-based hydrogels and I want to observe the microstructures (porosity, roughness, etc.). The hydrogels were produced by gelation in aqueous calcium chloride. SEM is out of...

30 August 2019 2,863 4 View

What is the best way to hold AFM cantilevers during piranha cleaning?

Hi, I'm trying to use piranha to clean cantilevers (silicon and gold coated SiNi) but I find that bubbles form on the cantilever chips and cause them to start to float or flip over, potentially...

24 March 2019 8,787 1 View

Is rice bran oil good for frying?

I am considering rice bran oil for frying to replace canola oil due to its benefits. I noticed difference in the consistency of the food, in this case the quality of caramel and its adhesion to...

29 May 2018 1,311 1 View

Can somebody assist me by giving me standards for fumonisins for my MSC project?

I am a Master of Biotechnology student at Kenyatta University, working on a project on determination of Fumonisin FB1, FB1, FB3 and FB4 on maize samples. I would like to use HPLC technique of...

30 October 2017 2,079 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View