Your research involving the sequential extraction of sediments using the Tessier et al. protocol is crucial for understanding the distribution of analytes in different sediment fractions. Regarding the determination of optimal experimental conditions for the extracts, there are several strategies you can consider:

Extraction Condition Optimization: Conduct optimization experiments for each solvent, varying factors such as extraction time, solid-liquid ratio, and the concentration of chelating agents in the case of chelating solvents like oxalic acid and ammonium oxalate monohydrate. The response of analytes in each fraction can be assessed using appropriate analytical techniques.

Experimental Design: Using an experimental design, such as response surface methodology, can systematically evaluate the influence of multiple variables on the extraction method's performance. This will allow you to identify optimal conditions and understand interactions between experimental factors.

Analytical Validation: Conducting analytical validation studies for each stage of the extraction procedure is crucial. This involves assessing linearity, precision, limit of detection, and quantification, among other parameters, to ensure the reliability of your results.

Regarding the measurement of metallic analytes in the solvents you mentioned, the United States Environmental Protection Agency (EPA) offers various standardized methods that could be useful. While there isn't a single method covering all the solvents you mentioned, some common EPA methods for determining metals in aqueous matrices and sediment extracts include atomic absorption spectrometry (AAS) and inductively coupled plasma mass spectrometry (ICP-MS).

It's important to review specific EPA methods related to your solvents and matrices to ensure you are using the most appropriate approach. Additionally, adapting existing methods or developing new methodologies may be necessary based on the specific characteristics of your research.

Sebastian Steven Beltran Jimenez

Thanks for your contribution. It is an eye-opener. I have tried to get some articles on the specific EPA methods related to those matrices mentioned but I could not get any yet. In case you have any, kindly share with me.

Thanks

Maybe this article can help you

Ptistišek, N., Milačič, R. & Veber, M. Use of the BCR three-step sequential extraction procedure for the study of the partitioning of Cd, Pb and Zn in various soil samples. J Soils & Sediments 1, 25–29 (2001). https://doi.org/10.1007/BF02986466

Sebastian Steven Beltran Jimenez Thanks for sharing