I am learning to barcode nematodes and was wondering which primer can be used to determine a species in the Bursaphelenchus genus. I do not need to maximize the phylogenetic relationship, just a pointer to a species identification.
Do iron salts increase photosynthesis, or do they increase the size of chloroplasts, or cytochromes when iron is sufficient? In other words, is greening-up functional or structural under these...
26 January 2021 9,584 4 View
I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...
12 January 2021 6,260 1 View
Greetings from Vancouver, Canada! My doctoral research is going to begin soon. Because the instance of higher education students in Canada, and elsewhere, has been on the rise for more than a...
11 November 2020 2,600 3 View
In an attempt to plot the michaelis- menten curve, it requires the initial rate as the y-axis and the substrate contration as the x-axis. Now how do you get the substrate concentration of the...
25 October 2020 1,702 3 View
I'm having difficulty recovering curcumin from my ethanolic extract. I tried reducing its solubility in the solution by slowly adding water, but the solid just crashed out of the solution in very...
03 September 2020 3,020 5 View
I am carrying out a research to assess the ecological risk associated with the speciation of trace and toxic metals in floodplain suspension. Bearing in mind that the reducible, oxidizable and...
18 August 2020 4,333 4 View
I'm working on alginate-based hydrogels and I want to observe the microstructures (porosity, roughness, etc.). The hydrogels were produced by gelation in aqueous calcium chloride. SEM is out of...
30 August 2019 2,863 4 View
Hi, I'm trying to use piranha to clean cantilevers (silicon and gold coated SiNi) but I find that bubbles form on the cantilever chips and cause them to start to float or flip over, potentially...
24 March 2019 8,787 1 View
I am considering rice bran oil for frying to replace canola oil due to its benefits. I noticed difference in the consistency of the food, in this case the quality of caramel and its adhesion to...
29 May 2018 1,311 1 View
I am a Master of Biotechnology student at Kenyatta University, working on a project on determination of Fumonisin FB1, FB1, FB3 and FB4 on maize samples. I would like to use HPLC technique of...
30 October 2017 2,079 3 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View
hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?
28 February 2021 1,254 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...
28 February 2021 400 1 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View