I am learning to barcode nematodes and was wondering which primer can be used to determine a species in the Bursaphelenchus genus. I do not need to maximize the phylogenetic relationship, just a pointer to a species identification.
The terms Fictional Language, Fictitious Language, Artificial Language, or Constructed Language in fiction has been used interchangeably in papers that I have read. Are there differences in these...
05 June 2024 3,453 7 View
relationship between public debt ,unemployement and economic growth
15 May 2024 3,927 3 View
We found any bubbles in DAF btw ASIC die and LF
14 March 2024 5,739 0 View
We are writing a thesis, and we need the Y-o-Y microfinance market growth rate of Vietnam, Philippines, Malaysia, Indonesia, and Thailand for 2013-2022. I already found the data for the...
18 February 2024 639 0 View
ChatGPT claims that the following general invertebrate primers are often used for nematode barcoding: Forward Primer: JB3: 5'-TTTTTTGGGCATCCTGAGGTTTAT-3' Reverse Primer: HCO2198:...
05 February 2024 6,008 4 View
I am trying to create a comprehensive list of the world's herpetology-focused research journals (extant and defunct). My only criteria are that the journals be peer-reviewed, non-predatory, and do...
19 January 2024 1,200 23 View
Nathan Cobb describes nematode lonema, as "Setae on head 4; eyes with lenses; amphid transverse-oval, at lips; among algae." What nematode anatomical structure has "eyes with lenses"? Or what is...
10 December 2023 4,223 1 View
I am compiling a list of resources that provide international nematology services (systematics, diagnostics, PCR, recommendations, remediation, or biosystematics). The entries will eventually be...
09 December 2023 9,042 0 View
Dear researchers, My research involves sequential extraction of sediments with different solvents (Acetic acid, Sodium acetate, Oxalic acid, and Ammonium oxalate monohydrate, Nitric acid) using...
19 November 2023 5,317 4 View
Dear researchers, The salt intake for the ICP-MS we have is < 0.2%. I have river water sample with salinity ranged from 11.79 to 12.51 g/L. What do you think can be done so that these water...
15 November 2023 4,744 6 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...
05 August 2024 1,238 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View