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Questions related from Lawrence Perepolkin
ChatGPT claims that the following general invertebrate primers are often used for nematode barcoding: Forward Primer: JB3: 5'-TTTTTTGGGCATCCTGAGGTTTAT-3' Reverse Primer: HCO2198:...
06 February 2024 5,964 4 View
I am learning to barcode nematodes and was wondering which primer can be used to determine a species in the Bursaphelenchus genus. I do not need to maximize the phylogenetic relationship, just a...
02 February 2024 5,148 0 View
Nathan Cobb describes nematode lonema, as "Setae on head 4; eyes with lenses; amphid transverse-oval, at lips; among algae." What nematode anatomical structure has "eyes with lenses"? Or what is...
11 December 2023 4,181 1 View
I am compiling a list of resources that provide international nematology services (systematics, diagnostics, PCR, recommendations, remediation, or biosystematics). The entries will eventually be...
10 December 2023 9,012 0 View
Under a microscope, both Allodorylaimus and Aporcelaimellus appear similar. Aporcelaimellus are plump nematodes. I can see different spears and lips.
20 June 2022 5,349 1 View
This is more of a job than fun -- tedious. I have been taking micrographs of nematodes extracted from various biogeoclimatic zones in southern British Columbia Canada. I would like to have...
04 June 2022 4,602 0 View
Has anyone translated nematode identification keys found in Tom Bongers' "De Nematoden van Nederland" from Dutch to English?
20 May 2022 8,190 0 View
Culturing nematodes and observing if they mate would be one. Are there other techniques? Would different reproductive systems imply different species?
16 May 2022 2,080 2 View
Diederich, J. & Milton, J., 1989. NEMISYS, an expert system for nematode identification. In: R. Fortuner (Ed.), Nematode identification and expert system technology, New York, Plenum Publishing...
14 December 2021 3,581 1 View
I observed a few nematodes and would like to have a select few of those observed identified at the species level. I was wondering which institutes or researchers in Canada can conclusively...
29 October 2021 8,500 3 View
Can members recommend protocols and primers for PCR identification of randomly selected nematodes? A general first sweep method for identification to family or genus. Can someone suggest some...
08 October 2021 8,526 4 View
I am looking for a detailed manual that one can follow as a self-teaching guide for measuring nematode morphometric parameters. Similar to architectural blueprints use in constructing buildings.
07 September 2021 9,653 6 View
To express the morphometric characteristics of the nematode better, De Man first suggested a system (1876). There have been since additions, modifications, and amendments made by Cobb (1914),...
30 August 2021 3,272 0 View
Can distinguishable proteins visible with fluorescent microscopy be used to taxonomically group and distinguish nematodes? Let nematode_1 have two fluorescent proteins and nematode_2 have two...
22 July 2019 9,728 1 View
I am thinking that Open Computer Vision can be used to facilitate the identification of nematodes. The application would need to be trained with known images of nematodes.
23 January 2019 7,811 3 View
Do there exist open data online sites where one can submit nematode micrographs, VCE videos, and image metadata? A content management system like Drupal could work. The level of effort and...
01 January 1970 161 1 View
With computer vision, a computer can with the help of an operator, perform accurate dimensional analysis of a nematode. The operator can also assist in the computer in identifying esophagus counts...
01 January 1970 1,195 10 View
Fiddling with some web presentation prototypes for West Kootenay Nematodes. Click on the link to view a female Chambersiellidae specimen. This prototype follows Paul De Ley's and Wim Bert's...
01 January 1970 3,966 0 View
