You can do the flow cytometry with neural specific antibody and can also do western blot.

Pls go thru this article  https://www.ncbi.nlm.nih.gov/pubmed/21618083 

Following articles might be helpful.,

1. http://pubs.acs.org/doi/abs/10.1021/acsami.6b06403 (DOI: 10.1021/acsami.6b06403)

2. http://www.sciencedirect.com/science/article/pii/S0142961215008881

You could also use some patch clump techniques to measure signal transmission and membrane potential to observe functional neurons.

Good luck

Hello

Elisa and  western blot its  good

http://www.hh.um.es/pdf/Vol_19/19_4/Bazan-19-1261-1275-2004.pdf

Hello Aya

You could perform immunohistochemistry against a pan-neuronal marker (= a protein that all neurons express), for example Tuj1 or HuC/D.

Good luck!