Aya Elgamal

15 Questions 4 Answers 0 Followers

Questions related from Aya Elgamal

Can anyone help me to get a protocol for cresyl violet acetate staining for cultured neural cells?

I undergo differentiation of stem cells into neural cells in 6 well plate and i want to do cresyl violet acetate staining for those cultured cells as a step to confirm differentation.

07 July 2018 2,303 8 View

What is the optimal method for preservation of CSF (cerebrospinal fluid sample) for usage in cell culture as a source of growth factor?

I collect multiple CSf samples and i want to preserve them until collection of all samples complete for usage in cell culture as a source of growth factor. How can i transport the sample? How can...

06 June 2018 5,511 3 View

What is the best anticoagulant (& its conc.)to prevent PRP/culture media from gel formation?

When i add PRP on the culture media it converted from liquid state into gel but i need it in the liquid state . how can i dissolve this problem!

03 March 2018 6,113 0 View

What are the different concentration of a new compound can i start on cancer cell line to calculate IC50?

I have a new compound and i want to calculate IC50 but i dont know what are the concentrations i must start with as a bilot study.

12 December 2017 3,848 0 View

What are the different concentration of a new compound can i start on cancer cell line to calculate IC50?

I have a new compound and i want to calculate IC50 but i dont know what are the concentrations i must start with as a bilot study.

12 December 2017 5,993 8 View

A simple Colorimetric method to measure cell viability for adherent cells in 96-well plates using dye /stain?

The usual methods for measuring cell viability in proliferation cytotoxicity assay is MTT / MTS /.......... I ask for another Inexpensive method using only stain for large number of tested samples.

12 December 2017 7,765 4 View

What is the mechanism of stem cell differentiation ?

I want to Know how stem cell differentiation occur for general either in vivo or in vitro and if this mechanism differ from one cell type to another or not.

04 April 2017 3,001 3 View

How to perform MTS assay on suspended cells like Ehrilch cells or lymphocyte?

i want to put MTS on suspended cells to evaluate viability but the data sheet demonstrate the details for attached cells but not demonstrate the fine detalis for suspended cells and there is some...

01 January 2017 7,943 2 View

How can calculate IC50 ?

I put different doses of certain drug on tumor cell , how can I choose the correct dose ( IC50 ) and draw the curve to complete my experiment.

12 December 2016 1,693 2 View

What are the difference between PRP and PRF and which method is preferable ?

I am confused between PRP and PRF Which is better!

10 October 2016 8,869 1 View

What are different methods for characterization of neural cells other than pcr or immunocytochemistry ?

my research work is depend on differentiation of mesenchymal stem cells into neurons,i need specific stain for neural cells to characterize it .

10 October 2016 9,236 6 View

What is the concentration of BMP9 for cholinergic neuron induction?

i want to make cholinergic neuron induction but what is the dose and time for this induction and is it adverse effect of it if time increse

03 March 2016 5,749 0 View

Can heparin solution (ampule) replace heparin powder on cell work?

There is a paper where heparin powder is used on cells. Can I use heparin solution ampule instead? What is the difference between heparin solution and heparin powder? Usually heparin ampules...

12 December 2015 6,405 1 View

What is the best method for neural induction ?

the best method for neural indution from mesenchymal stem cell

02 February 2015 3,922 1 View

Is sensation in rat , such as human or less, and Why ?

I do not know if sensation in rat ( lab animal) , such as human or lessThere is thought the answer but I do not know is true, not a mistake brain of human filled with gyri and sulci which...

02 February 2015 6,277 0 View