Ayşegül Doğan

3 Questions 6 Answers 0 Followers

Questions related from Ayşegül Doğan

How can I evaluate mouse tumor volume equivalents in human?

Can someone help me about mice to human conversions in the field of chemotherapy? I observed approximately 14 cm3 prostate cancer tumors in my control group animals (mice). However I do not know...

03 March 2015 8,130 5 View

What is the survival curve of 3T3-L1 preadipocytes for stable transfection?

I have transduced 3T3-L1 cells with lentiviral vectors expressing puromycin selection gene. Does anyone know the exact puromycin concentration for this cell line for selection?

11 November 2014 1,249 3 View

What are the important points for the detection of phospho-Akt in western blot?

I am trying to detect P-Akt in my samples but I generally observe non spesific binding. I am using cell signalling antibody (4058-dilution rate:1:5000). I have used 5 or 3% BSA for primary and...

10 October 2014 3,962 11 View