I undergo differentiation of stem cells into neural cells in 6 well plate and i want to do cresyl violet acetate staining for those cultured cells as a step to confirm differentation.
Hi
There are many on the web
https://openwetware.org/wiki/Crystal_Violet_Staining
Kunal Pal, did you notice how your answer is exactly the same as Farjam's answer?
Dear Dr. Farjam Goudarzi and Kunal Pal ,
Thanks alots for your answers but i asked about cresyl violet acetate staining not crystal Violet Staining
Both stainings are the same. Acetate is attached to the dye just for better solubility.
No. You are right about the ions, but additionally Crystal violet is used for gram staining, cresyl is used for Nissl.
My mistake. Thanks for the tip alberto. I used this one
http://www.ihcworld.com/_protocols/special_stains/nissl-frozen-section.htm
It also can be accompanied with luxol fast blue for meylin staining at the same time.
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