When i add PRP on the culture media it converted from liquid state into gel but i need it in the liquid state .
how can i dissolve this problem!
I undergo differentiation of stem cells into neural cells in 6 well plate and i want to do cresyl violet acetate staining for those cultured cells as a step to confirm differentation.
06 July 2018 2,316 8 View
I collect multiple CSf samples and i want to preserve them until collection of all samples complete for usage in cell culture as a source of growth factor. How can i transport the sample? How can...
05 June 2018 5,605 3 View
I have a new compound and i want to calculate IC50 but i dont know what are the concentrations i must start with as a bilot study.
11 December 2017 3,863 0 View
11 December 2017 6,043 8 View
The usual methods for measuring cell viability in proliferation cytotoxicity assay is MTT / MTS /.......... I ask for another Inexpensive method using only stain for large number of tested samples.
11 December 2017 7,777 4 View
I want to Know how stem cell differentiation occur for general either in vivo or in vitro and if this mechanism differ from one cell type to another or not.
03 April 2017 3,023 3 View
i want to put MTS on suspended cells to evaluate viability but the data sheet demonstrate the details for attached cells but not demonstrate the fine detalis for suspended cells and there is some...
31 December 2016 7,957 2 View
I put different doses of certain drug on tumor cell , how can I choose the correct dose ( IC50 ) and draw the curve to complete my experiment.
11 December 2016 1,714 2 View
I am confused between PRP and PRF Which is better!
09 October 2016 8,882 1 View
my research work is depend on differentiation of mesenchymal stem cells into neurons,i need specific stain for neural cells to characterize it .
09 October 2016 9,278 6 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View