Can anyone help me to get a protocol for cresyl violet acetate staining for cultured neural cells?

I undergo differentiation of stem cells into neural cells in 6 well plate and i want to do cresyl violet acetate staining for those cultured cells as a step to confirm differentation.

06 July 2018 2,237 8 View

What is the optimal method for preservation of CSF (cerebrospinal fluid sample) for usage in cell culture as a source of growth factor?

I collect multiple CSf samples and i want to preserve them until collection of all samples complete for usage in cell culture as a source of growth factor. How can i transport the sample? How can...

05 June 2018 5,454 3 View

What is the best anticoagulant (& its conc.)to prevent PRP/culture media from gel formation?

When i add PRP on the culture media it converted from liquid state into gel but i need it in the liquid state . how can i dissolve this problem!

02 March 2018 6,059 0 View

What are the different concentration of a new compound can i start on cancer cell line to calculate IC50?

I have a new compound and i want to calculate IC50 but i dont know what are the concentrations i must start with as a bilot study.

11 December 2017 3,793 0 View

What are the different concentration of a new compound can i start on cancer cell line to calculate IC50?

I have a new compound and i want to calculate IC50 but i dont know what are the concentrations i must start with as a bilot study.

11 December 2017 5,915 8 View

What is the mechanism of stem cell differentiation ?

I want to Know how stem cell differentiation occur for general either in vivo or in vitro and if this mechanism differ from one cell type to another or not.

03 April 2017 2,812 3 View

How to perform MTS assay on suspended cells like Ehrilch cells or lymphocyte?

31 December 2016 7,889 2 View

How can calculate IC50 ?

11 December 2016 1,639 2 View

What are the difference between PRP and PRF and which method is preferable ?

I am confused between PRP and PRF Which is better!

09 October 2016 8,814 1 View

What are different methods for characterization of neural cells other than pcr or immunocytochemistry ?

my research work is depend on differentiation of mesenchymal stem cells into neurons,i need specific stain for neural cells to characterize it .

09 October 2016 9,182 6 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View