Can anyone help me to get a protocol for cresyl violet acetate staining for cultured neural cells?

I undergo differentiation of stem cells into neural cells in 6 well plate and i want to do cresyl violet acetate staining for those cultured cells as a step to confirm differentation.

06 July 2018 2,237 8 View

What is the optimal method for preservation of CSF (cerebrospinal fluid sample) for usage in cell culture as a source of growth factor?

I collect multiple CSf samples and i want to preserve them until collection of all samples complete for usage in cell culture as a source of growth factor. How can i transport the sample? How can...

05 June 2018 5,454 3 View

What is the best anticoagulant (& its conc.)to prevent PRP/culture media from gel formation?

When i add PRP on the culture media it converted from liquid state into gel but i need it in the liquid state . how can i dissolve this problem!

02 March 2018 6,059 0 View

What are the different concentration of a new compound can i start on cancer cell line to calculate IC50?

I have a new compound and i want to calculate IC50 but i dont know what are the concentrations i must start with as a bilot study.

11 December 2017 3,793 0 View

What are the different concentration of a new compound can i start on cancer cell line to calculate IC50?

I have a new compound and i want to calculate IC50 but i dont know what are the concentrations i must start with as a bilot study.

11 December 2017 5,915 8 View

A simple Colorimetric method to measure cell viability for adherent cells in 96-well plates using dye /stain?

The usual methods for measuring cell viability in proliferation cytotoxicity assay is MTT / MTS /.......... I ask for another Inexpensive method using only stain for large number of tested samples.

11 December 2017 7,684 4 View

What is the mechanism of stem cell differentiation ?

I want to Know how stem cell differentiation occur for general either in vivo or in vitro and if this mechanism differ from one cell type to another or not.

03 April 2017 2,812 3 View

How to perform MTS assay on suspended cells like Ehrilch cells or lymphocyte?

31 December 2016 7,889 2 View

What are the difference between PRP and PRF and which method is preferable ?

I am confused between PRP and PRF Which is better!

09 October 2016 8,814 1 View

What are different methods for characterization of neural cells other than pcr or immunocytochemistry ?

my research work is depend on differentiation of mesenchymal stem cells into neurons,i need specific stain for neural cells to characterize it .

09 October 2016 9,182 6 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View