I want to isolate extracellular DNA from 1L of environmental sample (lake water). Is it possible to concentrate the ext. DNA in sample using anion exchange columns ? What are important consideration in attempting to do so?
I want to get as much as the flatness of e-beam evaporated Au on Si02/Si wafers. So I want to anneal au deposited Si02/Si wafers. The thickness of Si02 is 2nm. Is there any appropriate time and...
01 December 2020 7,696 3 View
I have expressed viral coat protein in BL21 cells. After purification of the protein, I am observing two bands, one of the size of the protein and one exact double size in SDS page. I think the...
01 June 2020 6,722 25 View
I would like to know what are the protocols, assays and theory to study a nanomaterial interaction with cancer stem cells (and not normal cancer cells).
15 December 2019 6,398 3 View
I was once told that stable isotopes of lighter elements such as H, N C , etc are found in stars, planets, etc. Can anyone suggest any literature which talks about the formation of these isotopes?
15 December 2019 6,535 3 View
Why vedic mathematics based algorithms for division, multiplication etc. are not seen in reputed journals of Algorithms, Hardware architectures.
01 May 2019 9,805 2 View
Do explain in term of BET based parameters such as BET Surface Area, Langmuir surface area, Adsorption-desorption volume of pores and pore diameter.
26 February 2019 9,079 5 View
Cancer cells uptake of NPs time period?
24 February 2019 6,710 5 View
There are lot of ways people are using drug delivery systems (DDS) to treat bone cancer, for instance: 1. Chemo drug loaded DDS 2. Gene DDS (mRNA) 3. Dual DDS 4. Drug- protein (growth factors,...
20 September 2018 1,591 3 View
Could someone tell me any specific cation which have a role to kill the tumor cells, without magnetic or photo-thermal use?
06 September 2018 1,219 3 View
The drug is loaded in nano-particles and the nature of drug is highly hydrophobic.
20 June 2018 1,633 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...
01 March 2021 2,512 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View