I want to isolate extracellular DNA from 1L of environmental sample (lake water). Is it possible to concentrate the ext. DNA in sample using anion exchange columns ? What are important consideration in attempting to do so?
What suggested changes can be brought in IFFCO so that, the rate of RTO can be reduced ?
03 June 2024 3,871 2 View
I am analyzing some transient absorption data for hydrogenated amorphous silicon. Its seems that the banggap is around 1.7 eV i.e. 730nm. The pump is at 350nm and the probe from 500-900nm. I have...
05 March 2024 9,861 1 View
I intend to perform mechanical tensile test for my polymer nanocomposites. Does there exists any ASTM standard to follow? Can I also know the dimensions of the specimen to be produced? Lastly, I...
12 March 2023 4,001 3 View
Phenol sulfonation will be carried at around 105 degrees celsius with concentrated sulfuric acid.
13 January 2023 9,435 4 View
Dear RG community and researchers, We are trying to grow biofilms in lab scale two-stage anaerobic (5 L) and aerobic (3 L) MBBR reactor (using media of 900 m2/m3 specific surface area) using an...
10 November 2022 2,151 0 View
Dear Researchers, I am building ML models (classification) for a dataset with a large number of positives (the ratio of positives to negatives is 90:10). I realize that accuracy may be a...
01 November 2022 3,000 3 View
While running g_mmpbsa, I gave commend in Linux Ubuntu, python MmPbSaStat.py -m energy_MM.xvg -p polar.xvg -a apolar.xvg (I have all python files) However it is telling Python command not...
02 October 2022 8,861 8 View
I am a little bit confused about the identification of grass species. Does anyone have grass identification-related paper or book with a picture please recommend me.
21 April 2022 3,513 2 View
Just like FSM, why pushdown automata not used in sequential logic design.
14 December 2020 8,274 7 View
I want to get as much as the flatness of e-beam evaporated Au on Si02/Si wafers. So I want to anneal au deposited Si02/Si wafers. The thickness of Si02 is 2nm. Is there any appropriate time and...
01 December 2020 8,029 3 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View