To transfect SH-Sy5Y cells, do we need to start a culture from new frozen stock and wait 3-4 passages every time before doing transfection?

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Fulvio Celsi

Hi Shruti. I've tried a couple of time to do like you said....but unfortunately results are not relaible...SH-SY5y (as the majority of cells) need at least 2-3 passages to stabilize..so..you need to wait! :) good luck

Shruti Bagla

Thanks for sharing your experience, I thought that would be the case, it just slows down everything... But thanks!!