We are interested in looking at vascularization and angiogenesis in some human brain samples. The ideal way would be to use immunostaining but our lab is not developed for IHC or any immunolabeling experiments. So I was wondering what would be a good marker to look at vascularization using qPCR? Any suggestions are welcome, and it would be best if you can also provide some citations. Thanks!
Robin Highley
Is western blotting an option? http://onlinelibrary.wiley.com/doi/10.1111/bpa.12331/abstract
Alternatively you could do Miller's alcian blue or EVG to look at vessels. IHC would be more robust though. It may be worth talking to your local histopathology lab who do CD31 routinely and may be happy to do it for you (for a price...)
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