Shruti Bagla

10 Questions 18 Answers 0 Followers

Questions related from Shruti Bagla

How to test mycoplasma contamination without further contamination of lab areas and pipettes?

Hello I have been asked to test mycoplasma contanimation in some cell culture samples by the adjacent lab using PCR. The lab has been getting their cultures tested outside and know they are...

09 September 2019 4,099 2 View

Can anyone help me with some sequencing analysis files?

Our lab outsourced whole exome sequencing to a company, and I received the analyzed files recently. I have always been a wet lab person, and do not understand most of the file types, and how to...

03 March 2018 6,648 5 View

What is the best marker to study Vascularization in Human cortex using RT-PCR?

We are interested in looking at vascularization and angiogenesis in some human brain samples. The ideal way would be to use immunostaining but our lab is not developed for IHC or any...

06 June 2016 3,495 2 View

Has anyone used the Biorad multiscreen apparatus with a 10-well comb/run?

Hi I wanted to use the Biorad or any other multiscreen apparatus to screen for multiple antibodies in a western blot membrane. I have about 8 samples so I have a 10-well comb with 8 lanes. I was...

09 September 2015 9,050 1 View

How do you use Quinolinic acid for cell culture treatment?

I want to design an experiment where we treat the neuroblastoma with quinolinic acid. Has anyone done this? Or treatment of primary cultures with QA? Where do you buy QA? And how do you suspend...

05 May 2015 5,010 2 View

How do you account for cell confluency when performing knock down studies in differentiated vs undifferentiated cells?

I am trying to knock down a gene of interest using siRNA in SH-SY5Y cells. But the amount of siRNA I use is mostly based on the well surface area etc. I use 50pmol/well in a 6-well plate. So is it...

03 March 2015 8,804 4 View

How can we isolate single-cells after transfection to generate stable SH-Sy5Y cell line? Also, do these cells grow well from a single cell?

I will be using CRIPSR genome editing to modify a gene and generate stable cell line. I intend to use a plasmid with puromycin selection marker to initially screen the cells. Does anyone has...

01 January 2015 296 7 View

When I start a culture of COS7 cells from stock, can we use the cells for transfection after growing them overnight?

I will be using COS7 cells to study some miRNA and gene interactions using Luciferase Assay. I need advice from people working with these cells. When I start a culture from stock, can we use the...

11 November 2014 8,590 4 View

Any suggestion for a transfection reagent for Sy5Y cells for siRNA transfection?

Hi, We have used FuGENE 6 (Promega) to transfect Sy5Y cells with plasmid DNA, but I just spoke to the promega tech support and they do not recommend FuGENE products or any of their transfection...

11 November 2014 3,772 3 View

To transfect SH-Sy5Y cells, do we need to start a culture from new frozen stock and wait 3-4 passages every time before doing transfection?

Can we not transfect the cells straight from Liquid N2 after culturing them overnight or something like that? Has anyone ever tried this?

10 October 2014 5,305 2 View