Fulvio Celsi

10 Questions 116 Answers 0 Followers

Questions related from Fulvio Celsi

CREB and p-CREB quantification?

I've come across a few publications were the authors to demonstrate phospho-CREB increase shows only the WB band of p-CREB without showing the total CREB. Is it correct ? because I always thought...

12 December 2016 1,625 3 View

Any suggestions in finding a Lab Host for TALENTs (European) grant?

Hello everyone! I would like to find a lab host to participate to TALENTs grant. It is a ìn european grant that provide salary for 18 months, divided in 12 to spent in an Host institution and 6 to...

08 August 2015 8,971 3 View

Which method can I use for protein precipitation from cell culture media and ELISA?

I'm trying to concentrate cell culture supernatant to evaluate levels of IL-1B trough ELISA. I would like to do with TCA-Acetone method. Do you have any good protocol? Also, I've read that...

04 April 2015 9,224 6 View

Hypertetraploid cell line, is possible to do gene editing?

Hi to all! I'm entering the field and so I have a lot of question..The first one is the model: We are currently working with a cell line that is Hypertetraployd , and I'm wondering if it could be...

10 October 2014 5,431 7 View

Any experience in transfecting Daoy cells?

Hi all, I'm starting to use Daoy cells (medulloblastoma), and I would need to transfect them. In literature seems that there is no info on that...Has anyone tried that? thanks

10 October 2014 466 5 View

How can I solve my BglII site destruction problem?

Hi all, I need to excise a sequence from a plasmid, but this sequence was cloned in a BglII site with BamHI sequence, destroying the BglII recognition sequence. Unfortunately, the new BamHI is...

03 March 2014 5,304 10 View

Can someone recommend the easiest way to activate Nf-kB in a cell line (simply HeLa)?

I guess serum-starvation is the simplest option, but for my project is not the best idea. Any other suggestions? Thanks in advance

07 July 2013 9,571 11 View

Intronic miRNA

I'm quite new in the field, and I'm looking for a software or (better) an online platform to analyze intronic sequences in my gene-of-interest, to discover if there are potential miRNA inside the...

01 January 2013 2,507 4 View

Human Microglia Cell Line

I'm looking for a human microglial cell line, any suggestion? I've read about the HMO6 cell line, produced by Nagai et al., but I cannot find if they are commercially available or not.

01 January 2013 9,662 55 View

Can I store the excised lane from the Blue Native Gel overnight? In which buffer? Is there any risk of protein diffusion?

My problem is that in theory, I have to make the first dimension (native) run and the second dimension (SDS-page) run on the same day, and then transfer on membrane still on the same day. Bu also...

12 December 2012 457 5 View