We recently ordered THLE-3 cells from ATCC. The flasks need to be coated with fibronectin, bovine serum albumin and collagen. ATCC uses bovine collagen type I. We have collagen type I isolated from rat tail in the lab. Just wondering if both are suitable for THLE-3 culture. Has anyone successfully cultured these cells with collagen type I from rat tail?
Naveen Kumar
Dear Moroski,
You can very well use the collagen from the rat tail but you must first know the concentration of collagen that has been isolated in your lab. as i remember you must use 0.01-0.1 percent collagen per mL. So if you are sure with your collagen concentration you can go ahead and optimize the procedure by trail and error ways..good luck
0 votes
0 thanks
- Badges
- Science method
More Catherine Moroski's questions See All
Similar questions and discussions