Hi, I have some whole ectoparasites to purify DNA and RNA in order to submit these samples to whole genomic sequencing.
Does anyone have some experience with good protocols for that?
Thanks!
Hello. I want to ask about the way to make ROI from other image. Please imagine streets and buildings. I have a multi-fluorescent microscopic image and have a figure for each color (red, green,...
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I seeded PBMCs in a 10 cm petri dish (1x10^8) and treated them with hM-CSF and incubated them at 37C with 5% CO2 for 3 days. After 3 days, I carefully added PBS to wash and lost most of my cells....
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Error [65]: Error, extent of vector too large or attribute table error." I uninstalled and reinstalled QGIS 3.26.2 but I still get the error. Any idea what is causing this?
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I am currently repeating the same procedures with slightly different methods, but every time, my brain tissue slices disappear from the slides within a few weeks of coverslipping them. The slides...
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Encyclopedia of Animal Cognition and Behavior Editors: Jennifer Vonk, Todd Shackelford
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As a final project for a college course, we need to do a primary culture of non parenquimatic liver cells from mice. In our first try we obtained a great amount of white blood cells instead of...
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I want to grow (in vitro) primary cells from different types of pediatric tumors. It is known that fetal bovine serum induces cell differentiation in embryonic tumors, mainly. What is the most...
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We are used the protocol for urease extration adaptated (to C. neoformans) from Amin et al.,2013: Briefly, the broth cultures were subjected to centrifugation (5,000 × g, 4 °C) and the...
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Hello, I want to obtain RNA from hypothalamus of rat. I planed protocol that I sliced brain into 100um by cryostat (-10℃) and punched out hypothlamus with brain puncher. However, I couldn't slice...
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In order to produce a chimeric antibody, I transformed HEK293T cells with two plasmids: one for heavy chain and the other for light chain IgG. The supernatant was purified in a Protein G-Sepharose...
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Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
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We are working with aquatic DNA samples and 7 different regions amplifying invertebrates, plants and vertebrates. We optimized our reactions in 96-well plates which worked well but now we do the...
09 May 2024 3,459 0 View
Hi everyone, I've been struggling to identify the family these guys belong to. Does anyone have any ideas? Taken from NE Atlantic 2200m depth.
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Freshwater shrimps of the genus Neocaridina which are kept in captivity (aquarium) have "dirty" gills (visible through the transparent carapace). The "parasite" was not motile. Mortality was...
22 February 2024 542 1 View
ChatGPT claims that the following general invertebrate primers are often used for nematode barcoding: Forward Primer: JB3: 5'-TTTTTTGGGCATCCTGAGGTTTAT-3' Reverse Primer: HCO2198:...
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See the attached figure below (from Zacks and Jablonka 2023). Notice that ‘sensory’ is segregated from ‘motor’ (except at the midbrain) just as it is for humans; in short, no sensory (and no...
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Has anyone come across experimental studies on the impact of guano on invertebrates? I'm referring to research, for instance, on the development of eggs or larvae. I vaguely recall seeing...
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Dear colleagues, We're desperately waiting for a reply from the RG support and Help Center, but our three requests left non-answered. There are two copies of the same our new paper...
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I am going to determine MK801 and DCS combined and alone effect on planaria, but I'm confused about it's dose. As in rodent MK801 doses 0.06, 0.03 mg /kg and DCS 25mg/kg but in planaria as I have...
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The crustacean order Tanaidacea consists of macrobenthic invertebrates, which constitute a major food source for higher trophic levels in estuarine and marine environment.
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