Can we dissolve plant proteins into human serum?

I want to check the presence of some specific antigenic proteins in a leaf. The system we have is used for detection of proteins using ELISA from serum samples. Is it possible that i crush my leaf...

03 April 2014 783 0 View

Can I use my plasmid as a positive control for ELISA?

I want to perform analysis on some plant samples for the detection of recombinant protein expression. For this purpose I want to use ELISA. Unfortunately I don't have a positive control. Is it...

02 March 2013 7,823 1 View

Multiple bands after PCR using nptII primer.

I am trying to detect putative transformed tomato plants by PCR using Kanamycin specific primer. Instead of getting one band of 720bp( primer designed for) I am getting multiple bands. I optimized...

07 August 2012 1,327 10 View

Southern blotting produced only a strong signal in the control but nothing in the test DNA. Did my transformation fail?

I transformed some plants, grown from initial stages till rooting on 100- 50 mg kanamycin media. After extracting DNA I did PCR using Gene specific primers and got clear sharp bands of desired...

05 June 2012 9,278 5 View

Labeling of southern probe by DIG PCR.

I am trying to label DNA probe by DIG PCR. First I successfully optimized the amplification of gene by simple PCR reagents. Then I used DIG components for PCR reaction mix as recommended by Roche...

04 May 2012 6,202 6 View

Problem in PCR

I designed some primers to amplify a tomato gene. When checked two primer pairs worked very well and gave me required length bands. I repeated this experiment three times and always get bands of...

01 February 2012 1,273 13 View

PCR product purification from gel.

Is there some easy manual( non kit) method to purify band from gel. Other than dialysis membrane and column elution. Thanks

01 February 2012 5,621 3 View

Purification of PCR product

Hello All, Can anyone have an experience to purify PCR product without using KITs suitable for ligation and cloning. If someone know any manual procedure please guide me about it. Thanks

01 February 2012 5,265 12 View

My question is that is there any way /method to check the function or activity of fruit specific promoter prior to transformation in plant?

I have isolated some fruit specific promoters by PCR amplification. On gel they are giving expected band size. I want to develop a gene cassette in which gene of interest will be under the control...

31 December 2011 1,666 6 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View