Prior to plant transformation, transformation in microbes is more feasible using vector. Functionality can be checked by using indicator gene downstream to the promoter under study and run the conditions in which the promoter allows transcription.

Thanks Prajakta Jadhav; I thougt about it before to place udiA(GUS) gene under the control of this promoter . This promoter express only in fruits. Do you think that it will work in bacteria? Please give me your view about this? Thanks

Since it's plant promoter and also the destined location is fruits so using bacteria might ruled out. But being an microbiologist would suggest to try for bacteria since its the simplest model to handle and study.

I can suggest u one way which can be tried,

If E.coli is an experimental system,

Construct an vector with 2 independent gene cassette...k

1. The promoter (P1) under study with indicator gene to its downstream...k

2. Lactose uitilization promoter with activator gene for promoter P1 (any activator which can be hormone).

Hope u get the above method and might work out.....

If transcription is an problem u can also go for simplest eukaryotes as model system....

If you have access to Arabidopsis I would suggest the following: Take your construction "your promoter::GUS" and perform an Arabidopsis floral dip transformation. In less than a month you will be able to check expression of GUS in the siliques (Arabidopsis fruit). Of course you should include a positive control (35Spromoter:GUS por example).

It might not work, but is simple, quite fast, and convincing if the result is positive.

Any other reporter gene (fluorescent proteins) are also a possibility.

Thanks Prajakta Jadhav, i will try this .

Virginia Garreton, Thanks for your kind answer. your suggestion is very nice. But i don't have any experience for floral dip method of transformation. How much time Arabidopsis will take to flower? Do you have experience about floral dip if yes then please guide me.