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Questions related from Sudeep Maurya
Hello everyone Is there any mouse cell line secreting CCL20/MIP3alpha? Can we use human CCL20 in mouse for its bio-activity or not?
08 August 2016 8,888 3 View
Can anyone suggest a eukaryotic linker for proteins with the property of auto cleavage or protease sensitive and size 3-6 amino acids.
04 April 2016 1,958 3 View
I am trying to express fusion peptides of mycobacterium tuberculosis in BL21DE3 and Rosseta DE3 but not getting any expression. Vector I have used is pET23a and pET28b. Gene is fusion of 6...
03 March 2016 7,361 5 View
I want to know that is there any possibility to clone complete promoter and ribosome binding site in a expression vector to increase the cloning site. I want to clone more than one gene to express...
11 November 2014 8,140 2 View
I would like to know whether the addition of OADC instead of ADC will impact the growth of mycobacterium.
09 September 2014 5,415 4 View
I want to express two mammalian gene into single plasmid of mycobacterium. In which way should these gene products should get secreted and separated? So which kind of strategy I should follow and...
08 August 2014 824 3 View
I am trying to ligate my plasmid digested with BamH l and Hind lll and same to my gene of interest but I am unable to ligate them. I have used alkaline phosphatase digestion for plasmid but still...
12 December 2013 9,790 13 View
I want to produce some Mtb peptide in high amount and want to clone in M. smegmatis. Can anyone suggest the best plasmid for cloning and high expression?
10 October 2012 4,981 0 View
