18 Questions 14 Answers 0 Followers
Questions related from Heena Jariyal
hello, Is there anyone who has sorted MCF-7 using CD24-/ CD44++/CD326++(epcam) markers. I got articles with CD24/44. Please share some articles with FACS profile from mcf-7 and MDAMB 231 with...
05 May 2018 2,397 0 View
Hello, everyone, I need plasmid for Lentiviral formation. Is there any lab in India which is using Lentiviral packaging plasmid. psPAX2 and pMD2.G. Please let us know.
09 September 2017 8,885 0 View
We are going to use SHC001 Scramble vector from sigma it does not contain any shRNA or GFP tag, however, it has ampicillin and puromycin resistance genes. In this case, how to characterize that...
09 September 2017 4,909 7 View
We have synthesized some inhibitors for PKM2 enzyme. we are planning to perform enzyme kinetic studies. we studied some protocols for the same but conc. of enzymes to be used are missing. some of...
09 September 2017 2,885 5 View
We are doing LDHA assy for mammalian cells. we are using conc enzyme .15 to .5 u/ml, pyruvate 2mM or NADH 20microM, for the standard curve but we are getting almost same absorbance. Please...
09 September 2017 6,108 0 View
I am trying to isolate cancer stem cells from MCF-7 and MDA MB231 with the help of tumorsphere assay. Is sorting necessary before tumorsphere assay cell seeding or do we sort cells after assay?...
09 September 2017 5,211 6 View
I want to do tumorsphere assay with MDAMB231 it is usually grown in L-15 or RMI 1640 media. So, do I need to prepare cancer stem cell CSCs specific media in L-15 or can I use CSCs media prepared...
08 August 2017 8,160 3 View
is there a major difference between sodium phosphate buffer and potassium phosphate buffer. can they be used alternatively?
07 July 2017 8,947 4 View
We are planning to buy shRNA for our experiments. can anyone suggest which form is good like whether one should buy bacterial stocks, plasmid or lentiviral particles? Is it good to buy bacterial...
07 July 2017 10,051 3 View
Hello, I want to study knockdown of a gene in cell. we are planing to buy it from sigma I have chosen the vector with shRNA insert but I am not able to understand how to choose scramble for this...
05 May 2017 8,382 1 View
Hello, I have isolated cancer stem cells from MCF-7 cells. I want to characterize these cells. Is there any option available other than FACS? Can we do it with PCR or western blot for markers of CSCs?
04 April 2017 371 5 View
Hello, I want to work with Cal148 Cell line of breast cancer but DSMZ (Germany) institute having it do not provide it in INDIA. ATCC is not having this cell line. Is there any other option from...
03 March 2017 530 1 View
Hello, I am isolation cancer stem cells from MCF-7 cells. I saw protocols for 96 well plate where 1 cell per well is seeded. How many cells should be seeded in 6 well plate.
03 March 2017 2,767 4 View
Hello, I wonder if we want to study a gene silencing in Cancer stem cells and we are doing a Knockdown in cancer cells. After a stable knockdown if we isolate cancer stem cells from same cell...
03 March 2017 1,977 2 View
Hello, I am working with breast cancer. I have read many articles in which they have isolated cells from cell lines. As the number of cancer stem cells is very less so is it possible to increase...
03 March 2017 3,765 10 View
Hello, I have chosen some cell lines on the bases of information available on cancer cell line encyclopedia but while my experiments I am not getting the same results . Can anyone tell how do they...
01 January 2017 9,070 5 View
i am growing MDA MB-468 cells in my lab. For few weeks we grow them in L-15 media but they were growing very slow , it took almost 20 days to become 25cm flask to get confluent . Now we shifted...
12 December 2016 8,628 7 View
I am working with MCF7 cell lines and want to check expression of Glutaminase 2 ( GLS2) . it is reported in one article that the cells have GLS2 expression. I had tried it at different annealing...
12 December 2016 3,533 1 View
